Detailed information of TA system TA040858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 59054..59625
• Replicon: plasmid pla1
• Accession: NZ_AP025755
• Organism: Escherichia sp. KS167_9B strain KS-P079

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B0VD50
• Locus tag: QUE34_RS26940
• Protein ID: WP_024166187.1
• Coordinates: 59344..59625 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: QUE34_RS26935
• Protein ID: WP_001081196.1
• Coordinates: 59054..59335 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE34_RS26910 (55444)	55444..55720	+	277	Protein_62	transposase	-
QUE34_RS26915 (55988)	55988..56992	-	1005	WP_001179711.1	IS110 family transposase	-
QUE34_RS26920 (57041)	57041..57999	+	959	Protein_64	IS3 family transposase	-
QUE34_RS26925 (58152)	58152..58805	+	654	Protein_65	helix-turn-helix domain-containing protein	-
QUE34_RS26930 (58806)	58806..58889	-	84	Protein_66	hypothetical protein	-
QUE34_RS26935 (59054)	59054..59335	-	282	WP_001081196.1	HigA family addiction module antitoxin	Antitoxin
QUE34_RS26940 (59344)	59344..59625	-	282	WP_024166187.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUE34_RS26945 (59790)	59790..61033	-	1244	Protein_69	group II intron reverse transcriptase/maturase	-
QUE34_RS26950 (61432)	61432..61610	+	179	Protein_70	hypothetical protein	-
QUE34_RS26955 (61614)	61614..61845	-	232	Protein_71	arylsulfatase regulator	-
QUE34_RS26960 (61868)	61868..62446	-	579	WP_000136193.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyD / hlyD / hlyB / hlyB / hlyA / hlyC / estIa / faeJ / faeI / faeH / faeF / faeE / faeD / faeC / espP	1..215429	215429
	-	inside	IScluster/Tn	-	-	33164..69049	35885

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-64)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10616.24 Da
• Isoelectric Point: 10.4126

>T40858 WP_024166187.1 NZ_AP025755:c59625-59344 [Escherichia sp. KS167_9B]
MIKSFRHKGLEHFFKTGSTAGIQAKHAVKLRIQLTALNMAKRPDDMSAPGWKLHPLKGADLKGHWAISVNGNWRLTFRFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T40858 NZ_AP025755:c59625-59344 [Escherichia sp. KS167_9B]
TTGATTAAGTCATTTCGCCATAAAGGGCTTGAACACTTTTTCAAGACAGGTTCAACCGCAGGCATTCAGGCCAAACATGC
GGTTAAGCTACGCATTCAATTAACCGCCCTTAATATGGCTAAACGCCCAGACGATATGTCTGCACCAGGCTGGAAATTAC
ACCCTCTGAAGGGAGCTGATCTTAAAGGCCATTGGGCTATATCTGTAAACGGTAACTGGCGACTTACCTTCCGATTTGAA
GGTGAAGACGCAATTCTGGTTGACTATCAGGATTATCATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10191.65 Da
• Isoelectric Point: 7.4026

>AT40858 WP_001081196.1 NZ_AP025755:c59335-59054 [Escherichia sp. KS167_9B]
MNRMHNPAHPGAVLREYLGEMSVTDAATALGITRTALSRILNEKAGISADMALRLESALGTSAEMWTGMQSQYELWVASQ
TARPNIRPLFHHA

Nucleotide

• Length: 282 bp

>AT40858 NZ_AP025755:c59335-59054 [Escherichia sp. KS167_9B]
ATGAATCGTATGCATAATCCCGCGCACCCAGGCGCGGTTCTTCGTGAATATCTGGGAGAAATGTCAGTCACAGACGCTGC
CACCGCATTAGGCATTACTCGCACGGCGCTTTCCCGGATTCTGAATGAAAAAGCTGGTATCTCTGCGGATATGGCGCTCC
GCCTGGAATCCGCACTCGGAACTAGCGCCGAAATGTGGACTGGTATGCAATCTCAGTATGAGCTTTGGGTTGCTTCGCAA
ACCGCTCGCCCAAACATTCGCCCATTATTTCACCATGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0B0VD50