Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1882142..1882704 Replicon chromosome
Accession NZ_AP025754
Organism Escherichia sp. KS167_9B strain KS-P079

Toxin (Protein)


Gene name higB Uniprot ID A0A2K3TT64
Locus tag QUE34_RS09460 Protein ID WP_032303016.1
Coordinates 1882426..1882704 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag QUE34_RS09455 Protein ID WP_000781370.1
Coordinates 1882142..1882426 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE34_RS09440 (1877501) 1877501..1880548 + 3048 WP_103253616.1 formate dehydrogenase-N subunit alpha -
QUE34_RS09445 (1880561) 1880561..1881445 + 885 WP_001240580.1 formate dehydrogenase N subunit beta -
QUE34_RS09450 (1881438) 1881438..1882091 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
QUE34_RS09455 (1882142) 1882142..1882426 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
QUE34_RS09460 (1882426) 1882426..1882704 - 279 WP_032303016.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QUE34_RS09465 (1882890) 1882890..1883900 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
QUE34_RS09470 (1884035) 1884035..1885732 - 1698 WP_032303017.1 malate dehydrogenase -
QUE34_RS09475 (1885889) 1885889..1886026 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
QUE34_RS09480 (1886128) 1886128..1886343 - 216 WP_000495766.1 biofilm-dependent modulation protein -
QUE34_RS09485 (1886688) 1886688..1887119 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10535.13 Da        Isoelectric Point: 7.3562

>T40840 WP_032303016.1 NZ_AP025754:c1882704-1882426 [Escherichia sp. KS167_9B]
MIMNFRHKGLRDLFLLGKTSGVIPTQIKRLRHRLAVIDAASCLADLDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T40840 NZ_AP025754:c1882704-1882426 [Escherichia sp. KS167_9B]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAAT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCTGATCTCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGTGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT40840 WP_000781370.1 NZ_AP025754:c1882426-1882142 [Escherichia sp. KS167_9B]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT40840 NZ_AP025754:c1882426-1882142 [Escherichia sp. KS167_9B]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAGCTGAACGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCCGCGTTGACGCCAGAAATGGCGA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTACAAAACGCCTGGAGTCTGGCAGAGGCAGAA
AAAACGGTAGATGTGTCGCGACTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K3TT64

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References