Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 44182..44446 Replicon plasmid pla2
Accession NZ_AP025753
Organism Escherichia sp. HH154_1D strain HH-P049

Toxin (Protein)


Gene name pndA Uniprot ID E6BRV3
Locus tag QUE53_RS27825 Protein ID WP_001303307.1
Coordinates 44294..44446 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 44182..44239 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE53_RS27810 (39421) 39421..41712 - 2292 WP_072119433.1 F-type conjugative transfer protein TrbC -
QUE53_RS27815 (41705) 41705..42775 - 1071 WP_000151580.1 IncI1-type conjugal transfer protein TrbB -
QUE53_RS27820 (42794) 42794..44002 - 1209 WP_001303305.1 IncI1-type conjugal transfer protein TrbA -
- (44182) 44182..44239 - 58 NuclAT_0 - Antitoxin
- (44182) 44182..44239 - 58 NuclAT_0 - Antitoxin
- (44182) 44182..44239 - 58 NuclAT_0 - Antitoxin
- (44182) 44182..44239 - 58 NuclAT_0 - Antitoxin
QUE53_RS27825 (44294) 44294..44446 + 153 WP_001303307.1 Hok/Gef family protein Toxin
QUE53_RS27830 (44518) 44518..44769 - 252 WP_001291967.1 hypothetical protein -
QUE53_RS27835 (45268) 45268..45363 + 96 WP_001303310.1 DinQ-like type I toxin DqlB -
QUE53_RS27840 (45428) 45428..45604 - 177 WP_001054898.1 hypothetical protein -
QUE53_RS27845 (45936) 45936..46145 + 210 WP_000062602.1 HEAT repeat domain-containing protein -
QUE53_RS27850 (46217) 46217..46879 - 663 WP_000653333.1 plasmid IncI1-type surface exclusion protein ExcA -
QUE53_RS27855 (46944) 46944..49106 - 2163 WP_032220734.1 DotA/TraY family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..91132 91132


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5817.21 Da        Isoelectric Point: 8.7948

>T40820 WP_001303307.1 NZ_AP025753:44294-44446 [Escherichia sp. HH154_1D]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T40820 NZ_AP025753:44294-44446 [Escherichia sp. HH154_1D]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 58 bp

>AT40820 NZ_AP025753:c44239-44182 [Escherichia sp. HH154_1D]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A634Q5L1

Antitoxin

Download structure file

References