Detailed information of TA system TA040815

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 125729..126155
• Replicon: plasmid pla1
• Accession: NZ_AP025752
• Organism: Escherichia sp. HH154_1D strain HH-P049

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: QUE53_RS27020
• Protein ID: WP_001372321.1
• Coordinates: 125729..125854 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	125931..126155 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE53_RS26990 (121282)	121282..121929	+	648	WP_000614283.1	transglycosylase SLT domain-containing protein	-
QUE53_RS26995 (122226)	122226..123047	-	822	Protein_148	DUF932 domain-containing protein	-
QUE53_RS27000 (123166)	123166..123453	-	288	WP_000107535.1	hypothetical protein	-
QUE53_RS27005 (123704)	123704..124708	+	1005	WP_024166184.1	IS110 family transposase	-
QUE53_RS27010 (124802)	124802..125062	-	261	WP_000547943.1	hypothetical protein	-
QUE53_RS27015 (125132)	125132..125304	+	173	Protein_152	hypothetical protein	-
QUE53_RS27020 (125729)	125729..125854	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
QUE53_RS27025 (125796)	125796..125945	-	150	Protein_154	plasmid maintenance protein Mok	-
- (125931)	125931..126155	-	225	NuclAT_0	-	Antitoxin
- (125931)	125931..126155	-	225	NuclAT_0	-	Antitoxin
- (125931)	125931..126155	-	225	NuclAT_0	-	Antitoxin
- (125931)	125931..126155	-	225	NuclAT_0	-	Antitoxin
QUE53_RS27030 (125967)	125967..126155	+	189	WP_001355331.1	hypothetical protein	-
QUE53_RS27035 (126100)	126100..126886	-	787	Protein_156	plasmid SOS inhibition protein A	-
QUE53_RS27040 (126883)	126883..127317	-	435	WP_000845944.1	conjugation system SOS inhibitor PsiB	-
QUE53_RS27045 (127386)	127386..129350	-	1965	WP_000117199.1	ParB/RepB/Spo0J family partition protein	-
QUE53_RS27050 (129414)	129414..129647	-	234	WP_000005990.1	DUF905 family protein	-
QUE53_RS27055 (129705)	129705..130244	-	540	WP_000290857.1	single-stranded DNA-binding protein	-
QUE53_RS27060 (130628)	130628..130885	-	258	WP_023147917.1	hypothetical protein	-
QUE53_RS27065 (130798)	130798..131011	-	214	Protein_162	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyD / hlyD / hlyB / hlyB / hlyA / hlyA / hlyC / estIa / faeJ / faeI / faeH / faeF / faeE / faeD / faeC / espP	1..220055	220055

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T40815 WP_001372321.1 NZ_AP025752:c125854-125729 [Escherichia sp. HH154_1D]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T40815 NZ_AP025752:c125854-125729 [Escherichia sp. HH154_1D]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT40815 NZ_AP025752:c126155-125931 [Escherichia sp. HH154_1D]
TCACACGGATTGCCCGTGAACTGGCTGAACGACAAGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGTAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin