Detailed information of TA system TA040809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 59702..60273
• Replicon: plasmid pla1
• Accession: NZ_AP025752
• Organism: Escherichia sp. HH154_1D strain HH-P049

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B0VD50
• Locus tag: QUE53_RS26600
• Protein ID: WP_024166187.1
• Coordinates: 59992..60273 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: QUE53_RS26595
• Protein ID: WP_001081196.1
• Coordinates: 59702..59983 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE53_RS26570 (56092)	56092..56368	+	277	Protein_63	transposase	-
QUE53_RS26575 (56636)	56636..57640	-	1005	WP_001179711.1	IS110 family transposase	-
QUE53_RS26580 (57689)	57689..58647	+	959	Protein_65	IS3 family transposase	-
QUE53_RS26585 (58800)	58800..59453	+	654	Protein_66	helix-turn-helix domain-containing protein	-
QUE53_RS26590 (59454)	59454..59537	-	84	Protein_67	hypothetical protein	-
QUE53_RS26595 (59702)	59702..59983	-	282	WP_001081196.1	HigA family addiction module antitoxin	Antitoxin
QUE53_RS26600 (59992)	59992..60273	-	282	WP_024166187.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUE53_RS26605 (60438)	60438..61681	-	1244	Protein_70	group II intron reverse transcriptase/maturase	-
QUE53_RS26610 (62080)	62080..62258	+	179	Protein_71	hypothetical protein	-
QUE53_RS26615 (62262)	62262..62493	-	232	Protein_72	arylsulfatase regulator	-
QUE53_RS26620 (62516)	62516..63094	-	579	WP_000136193.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyD / hlyD / hlyB / hlyB / hlyA / hlyA / hlyC / estIa / faeJ / faeI / faeH / faeF / faeE / faeD / faeC / espP	1..220055	220055
	-	inside	IScluster/Tn	-	-	33160..69697	36537

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-64)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10616.24 Da
• Isoelectric Point: 10.4126

>T40809 WP_024166187.1 NZ_AP025752:c60273-59992 [Escherichia sp. HH154_1D]
MIKSFRHKGLEHFFKTGSTAGIQAKHAVKLRIQLTALNMAKRPDDMSAPGWKLHPLKGADLKGHWAISVNGNWRLTFRFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T40809 NZ_AP025752:c60273-59992 [Escherichia sp. HH154_1D]
TTGATTAAGTCATTTCGCCATAAAGGGCTTGAACACTTTTTCAAGACAGGTTCAACCGCAGGCATTCAGGCCAAACATGC
GGTTAAGCTACGCATTCAATTAACCGCCCTTAATATGGCTAAACGCCCAGACGATATGTCTGCACCAGGCTGGAAATTAC
ACCCTCTGAAGGGAGCTGATCTTAAAGGCCATTGGGCTATATCTGTAAACGGTAACTGGCGACTTACCTTCCGATTTGAA
GGTGAAGACGCAATTCTGGTTGACTATCAGGATTATCATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10191.65 Da
• Isoelectric Point: 7.4026

>AT40809 WP_001081196.1 NZ_AP025752:c59983-59702 [Escherichia sp. HH154_1D]
MNRMHNPAHPGAVLREYLGEMSVTDAATALGITRTALSRILNEKAGISADMALRLESALGTSAEMWTGMQSQYELWVASQ
TARPNIRPLFHHA

Nucleotide

• Length: 282 bp

>AT40809 NZ_AP025752:c59983-59702 [Escherichia sp. HH154_1D]
ATGAATCGTATGCATAATCCCGCGCACCCAGGCGCGGTTCTTCGTGAATATCTGGGAGAAATGTCAGTCACAGACGCTGC
CACCGCATTAGGCATTACTCGCACGGCGCTTTCCCGGATTCTGAATGAAAAAGCTGGTATCTCTGCGGATATGGCGCTCC
GCCTGGAATCCGCACTCGGAACTAGCGCCGAAATGTGGACTGGTATGCAATCTCAGTATGAGCTTTGGGTTGCTTCGCAA
ACCGCTCGCCCAAACATTCGCCCATTATTTCACCATGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0B0VD50