Detailed information of TA system TA040803

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3345116..3345843
• Replicon: chromosome
• Accession: NZ_AP025751
• Organism: Escherichia sp. HH154_1D strain HH-P049

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0L6ZX69
• Locus tag: QUE53_RS16595
• Protein ID: WP_000547560.1
• Coordinates: 3345532..3345843 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUE53_RS16590
• Protein ID: WP_000126290.1
• Coordinates: 3345116..3345535 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE53_RS16570 (3340164)	3340164..3340691	-	528	WP_001078777.1	electron transport protein HydN	-
QUE53_RS16575 (3340841)	3340841..3341851	-	1011	WP_032193944.1	DNA-binding transcriptional regulator AscG	-
QUE53_RS16580 (3342111)	3342111..3343568	+	1458	WP_001107903.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
QUE53_RS16585 (3343577)	3343577..3345001	+	1425	WP_000110369.1	6-phospho-beta-glucosidase AscB	-
QUE53_RS16590 (3345116)	3345116..3345535	-	420	WP_000126290.1	helix-turn-helix domain-containing protein	Antitoxin
QUE53_RS16595 (3345532)	3345532..3345843	-	312	WP_000547560.1	type II toxin-antitoxin system HigB family toxin	Toxin
QUE53_RS16600 (3346011)	3346011..3346481	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
QUE53_RS16605 (3346474)	3346474..3346884	-	411	WP_001291923.1	formate hydrogenlyase assembly protein HycH	-
QUE53_RS16610 (3346881)	3346881..3347648	-	768	WP_000067390.1	formate hydrogenlyase subunit HycG	-
QUE53_RS16615 (3347648)	3347648..3348190	-	543	WP_000493798.1	formate hydrogenlyase subunit HycF	-
QUE53_RS16620 (3348200)	3348200..3349909	-	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12423.12 Da
• Isoelectric Point: 9.7106

>T40803 WP_000547560.1 NZ_AP025751:c3345843-3345532 [Escherichia sp. HH154_1D]
MHIISKAPFEESARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T40803 NZ_AP025751:c3345843-3345532 [Escherichia sp. HH154_1D]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAATAAATGGTACGTTCTGGATGTAGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCATGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15453.48 Da
• Isoelectric Point: 4.5628

>AT40803 WP_000126290.1 NZ_AP025751:c3345535-3345116 [Escherichia sp. HH154_1D]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNHEKFAEFDKAVAAMPI
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT40803 NZ_AP025751:c3345535-3345116 [Escherichia sp. HH154_1D]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGTATTGCAGAATATGAAAACAACCACGAAAAGTTTGCAGAGTTCGATAAAGCCGTTGCAGCAATGCCGATT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAAAGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0L6ZX69