Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1871104..1871666 Replicon chromosome
Accession NZ_AP025751
Organism Escherichia sp. HH154_1D strain HH-P049

Toxin (Protein)


Gene name higB Uniprot ID E9XKD5
Locus tag QUE53_RS09265 Protein ID WP_000605673.1
Coordinates 1871388..1871666 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag QUE53_RS09260 Protein ID WP_000781370.1
Coordinates 1871104..1871388 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE53_RS09245 (1866286) 1866286..1869333 + 3048 WP_015953395.1 formate dehydrogenase-N subunit alpha -
QUE53_RS09250 (1869346) 1869346..1870230 + 885 WP_001240580.1 formate dehydrogenase N subunit beta -
QUE53_RS09255 (1870223) 1870223..1870876 + 654 WP_000045648.1 formate dehydrogenase-N subunit gamma -
QUE53_RS09260 (1871104) 1871104..1871388 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
QUE53_RS09265 (1871388) 1871388..1871666 - 279 WP_000605673.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QUE53_RS09270 (1871852) 1871852..1872862 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
QUE53_RS09275 (1872997) 1872997..1874694 - 1698 WP_000433464.1 malate dehydrogenase -
QUE53_RS09280 (1874851) 1874851..1874988 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
QUE53_RS09285 (1875090) 1875090..1875305 - 216 WP_000495766.1 biofilm-dependent modulation protein -
QUE53_RS09290 (1875649) 1875649..1876080 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10535.13 Da        Isoelectric Point: 7.3562

>T40793 WP_000605673.1 NZ_AP025751:c1871666-1871388 [Escherichia sp. HH154_1D]
MIMNFRHKGLRDLFLLGKTSGVIPTQIKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T40793 NZ_AP025751:c1871666-1871388 [Escherichia sp. HH154_1D]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAAT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGTGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT40793 WP_000781370.1 NZ_AP025751:c1871388-1871104 [Escherichia sp. HH154_1D]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT40793 NZ_AP025751:c1871388-1871104 [Escherichia sp. HH154_1D]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAGCTGAACGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCCGCGTTGACGCCAGAAATGGCGA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTACAAAACGCCTGGAGTCTGGCAGAGGCAGAA
AAAACGGTAGATGTGTCGCGATTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7ADF6

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References