Detailed information of TA system TA040780

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2500..3146
• Replicon: plasmid pla2
• Accession: NZ_AP025749
• Organism: Escherichia sp. HH091_1A strain HH-P024

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: QUF14_RS28475
• Protein ID: WP_000269912.1
• Coordinates: 2500..2847 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUF14_RS28480
• Protein ID: WP_001259435.1
• Coordinates: 2847..3146 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF14_RS28460 (EsCd1HHP024_05700)	189..1037	+	849	WP_225190326.1	plasmid replication initiator RepA	-
QUF14_RS28465 (EsCd1HHP024_05701)	1681..1968	+	288	WP_000356589.1	hypothetical protein	-
QUF14_RS28470 (EsCd1HHP024_05702)	1992..2255	+	264	WP_000424604.1	hypothetical protein	-
QUF14_RS28475 (EsCd1HHP024_05703)	2500..2847	+	348	WP_000269912.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUF14_RS28480 (EsCd1HHP024_05704)	2847..3146	+	300	WP_001259435.1	XRE family transcriptional regulator	Antitoxin
QUF14_RS28485 (EsCd1HHP024_05705)	3310..3690	+	381	WP_000061761.1	hypothetical protein	-
QUF14_RS28490 (EsCd1HHP024_05706)	3794..4219	-	426	WP_016240784.1	hypothetical protein	-
QUF14_RS28495 (EsCd1HHP024_05707)	4321..4593	+	273	WP_042038402.1	helix-turn-helix domain-containing protein	-
QUF14_RS28500 (EsCd1HHP024_05708)	5204..5392	+	189	WP_004105254.1	hypothetical protein	-
QUF14_RS28505	5403..6119	-	717	WP_223673534.1	Rha family transcriptional regulator	-
QUF14_RS28510	6198..6545	-	348	Protein_10	ORF6N domain-containing protein	-
QUF14_RS28515 (EsCd1HHP024_05710)	6542..6733	-	192	WP_042038396.1	hypothetical protein	-
QUF14_RS28520	6847..7002	-	156	Protein_12	ash family protein	-
QUF14_RS28525 (EsCd1HHP024_05711)	7863..8111	-	249	WP_000730008.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..48993	48993

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13545.51 Da
• Isoelectric Point: 8.8337

>T40780 WP_000269912.1 NZ_AP025749:2500-2847 [Escherichia sp. HH091_1A]
MWTVLFSQRFDDWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYEKLVRIAEDEFAAHLNTLESK

Nucleotide

• Length: 348 bp

>T40780 NZ_AP025749:2500-2847 [Escherichia sp. HH091_1A]
ATGTGGACGGTACTTTTTAGCCAGCGATTTGACGACTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGCCGGGCCATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAGCGGTTTTATGAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11046.87 Da
• Isoelectric Point: 5.2090

>AT40780 WP_001259435.1 NZ_AP025749:2847-3146 [Escherichia sp. HH091_1A]
MRTLDEAIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRIAFNI

Nucleotide

• Length: 300 bp

>AT40780 NZ_AP025749:2847-3146 [Escherichia sp. HH091_1A]
ATGAGAACATTAGATGAGGCGATTGCCAGCCGTTCACCGGAAAGCCAGGCGCGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAACAGGTTGCTGAGGCGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACATTAAAGCGTTACGTTGAAGCTATGGGA
GGCAAATTAAGCCTGGATGTTGAGCTTCCCACAGGAAAACGAATAGCGTTTAACATCTGA