Detailed information of TA system TA040775

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 105797..106223
• Replicon: plasmid pla1
• Accession: NZ_AP025748
• Organism: Escherichia sp. HH091_1A strain HH-P024

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: QUF14_RS27765
• Protein ID: WP_001372321.1
• Coordinates: 105797..105922 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	105999..106223 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF14_RS27745 (100807)	100807..102711	-	1905	WP_236927347.1	type-F conjugative transfer system mating-pair stabilization protein TraN	-
QUF14_RS27750 (102769)	102769..103446	+	678	WP_100733974.1	IS66-like element accessory protein TnpA	-
QUF14_RS27755 (103446)	103446..103793	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
QUF14_RS27760 (103813)	103813..105384	+	1572	WP_000381395.1	IS66-like element ISCro1 family transposase	-
QUF14_RS27765 (105797)	105797..105922	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
QUF14_RS27770 (105864)	105864..106013	-	150	Protein_126	plasmid maintenance protein Mok	-
- (105999)	105999..106223	-	225	NuclAT_0	-	Antitoxin
- (105999)	105999..106223	-	225	NuclAT_0	-	Antitoxin
- (105999)	105999..106223	-	225	NuclAT_0	-	Antitoxin
- (105999)	105999..106223	-	225	NuclAT_0	-	Antitoxin
QUF14_RS27775 (106035)	106035..106223	+	189	WP_001355331.1	hypothetical protein	-
QUF14_RS27780 (106168)	106168..106954	-	787	Protein_128	plasmid SOS inhibition protein A	-
QUF14_RS27785 (106951)	106951..107385	-	435	WP_000845944.1	conjugation system SOS inhibitor PsiB	-
QUF14_RS27790 (107454)	107454..109418	-	1965	Protein_130	ParB/RepB/Spo0J family partition protein	-
QUF14_RS27795 (109482)	109482..109715	-	234	WP_000005990.1	DUF905 family protein	-
QUF14_RS27800 (109773)	109773..110312	-	540	WP_047088942.1	single-stranded DNA-binding protein	-
QUF14_RS27805 (110398)	110398..110544	-	147	Protein_133	single-stranded DNA-binding protein	-
QUF14_RS27810 (110784)	110784..111041	-	258	WP_023147917.1	hypothetical protein	-
QUF14_RS27815 (110954)	110954..111167	-	214	Protein_135	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	estIa / estIa / hlyC / hlyA / hlyB / hlyD / exeG / exeE / stcE / faeJ / faeI / faeH / faeF / faeE / faeD / faeC	1..225721	225721

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T40775 WP_001372321.1 NZ_AP025748:c105922-105797 [Escherichia sp. HH091_1A]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T40775 NZ_AP025748:c105922-105797 [Escherichia sp. HH091_1A]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT40775 NZ_AP025748:c106223-105999 [Escherichia sp. HH091_1A]
TCACACGGATTGCCCGTGAACTGGCTGAACGACAAGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGTAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin