Detailed information of TA system TA040769

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 60097..60668
• Replicon: plasmid pla1
• Accession: NZ_AP025748
• Organism: Escherichia sp. HH091_1A strain HH-P024

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B0VD50
• Locus tag: QUF14_RS27505
• Protein ID: WP_024166187.1
• Coordinates: 60387..60668 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: QUF14_RS27500
• Protein ID: WP_001081196.1
• Coordinates: 60097..60378 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF14_RS27470 (55172)	55172..55448	+	277	Protein_66	transposase	-
QUF14_RS27475 (55716)	55716..56711	-	996	WP_286333589.1	IS110 family transposase	-
QUF14_RS27480 (56765)	56765..57978	+	1214	WP_163451661.1	IS3 family transposase	-
QUF14_RS27485 (58082)	58082..59040	+	959	Protein_69	IS3 family transposase	-
QUF14_RS27490 (59193)	59193..59847	+	655	Protein_70	helix-turn-helix domain-containing protein	-
QUF14_RS27495 (59848)	59848..59931	-	84	Protein_71	hypothetical protein	-
QUF14_RS27500 (60097)	60097..60378	-	282	WP_001081196.1	HigA family addiction module antitoxin	Antitoxin
QUF14_RS27505 (60387)	60387..60668	-	282	WP_024166187.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUF14_RS27510 (60808)	60808..61362	-	555	Protein_74	IS21-like element ISEc10 family helper ATPase IstB	-
QUF14_RS27515 (61358)	61358..62140	-	783	Protein_75	hypothetical protein	-
QUF14_RS27520 (62189)	62189..62344	-	156	WP_000074142.1	hypothetical protein	-
QUF14_RS27525 (63009)	63009..64157	-	1149	WP_000586939.1	quinone-dependent L-lactate dehydrogenase	-
QUF14_RS27530 (64154)	64154..64930	-	777	WP_000217412.1	transcriptional regulator LldR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	estIa / estIa / hlyC / hlyA / hlyB / hlyD / exeG / exeE / stcE / faeJ / faeI / faeH / faeF / faeE / faeD / faeC	1..225721	225721
	-	inside	IScluster/Tn	-	-	36824..61326	24502

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-64)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10616.24 Da
• Isoelectric Point: 10.4126

>T40769 WP_024166187.1 NZ_AP025748:c60668-60387 [Escherichia sp. HH091_1A]
MIKSFRHKGLEHFFKTGSTAGIQAKHAVKLRIQLTALNMAKRPDDMSAPGWKLHPLKGADLKGHWAISVNGNWRLTFRFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T40769 NZ_AP025748:c60668-60387 [Escherichia sp. HH091_1A]
TTGATTAAGTCATTTCGCCATAAAGGGCTTGAACACTTTTTCAAGACAGGTTCAACCGCAGGCATTCAGGCCAAACATGC
GGTTAAGCTACGCATTCAATTAACCGCCCTTAATATGGCTAAACGCCCAGACGATATGTCTGCACCAGGCTGGAAATTAC
ACCCTCTGAAGGGAGCTGATCTTAAAGGCCATTGGGCTATATCTGTAAACGGTAACTGGCGACTTACCTTCCGATTTGAA
GGTGAAGACGCAATTCTGGTTGACTATCAGGATTATCATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10191.65 Da
• Isoelectric Point: 7.4026

>AT40769 WP_001081196.1 NZ_AP025748:c60378-60097 [Escherichia sp. HH091_1A]
MNRMHNPAHPGAVLREYLGEMSVTDAATALGITRTALSRILNEKAGISADMALRLESALGTSAEMWTGMQSQYELWVASQ
TARPNIRPLFHHA

Nucleotide

• Length: 282 bp

>AT40769 NZ_AP025748:c60378-60097 [Escherichia sp. HH091_1A]
ATGAATCGTATGCATAATCCCGCGCACCCAGGCGCGGTTCTTCGTGAATATCTGGGAGAAATGTCAGTCACAGACGCTGC
CACCGCATTAGGCATTACTCGCACGGCGCTTTCCCGGATTCTGAATGAAAAAGCTGGTATCTCTGCGGATATGGCGCTCC
GCCTGGAATCCGCACTCGGAACTAGCGCCGAAATGTGGACTGGTATGCAATCTCAGTATGAGCTTTGGGTTGCTTCGCAA
ACCGCTCGCCCAAACATTCGCCCATTATTTCACCATGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0B0VD50