Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3873807..3874534 Replicon chromosome
Accession NZ_AP025747
Organism Escherichia sp. HH091_1A strain HH-P024

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag QUF14_RS19435 Protein ID WP_000550189.1
Coordinates 3874220..3874534 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag QUF14_RS19430 Protein ID WP_000560267.1
Coordinates 3873807..3874223 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUF14_RS19420 (3869069) 3869069..3871420 + 2352 WP_000695523.1 alpha-glucosidase -
QUF14_RS19425 (3871744) 3871744..3873762 + 2019 WP_100733924.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
QUF14_RS19430 (3873807) 3873807..3874223 - 417 WP_000560267.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
QUF14_RS19435 (3874220) 3874220..3874534 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
QUF14_RS19440 (3874762) 3874762..3875898 - 1137 WP_000018674.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
QUF14_RS19445 (3875983) 3875983..3876486 + 504 WP_024212170.1 M48 family metallopeptidase -
QUF14_RS19450 (3876563) 3876563..3877255 + 693 WP_000942563.1 vancomycin high temperature exclusion protein -
QUF14_RS19455 (3877334) 3877334..3878320 + 987 WP_100733925.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T40763 WP_000550189.1 NZ_AP025747:c3874534-3874220 [Escherichia sp. HH091_1A]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T40763 NZ_AP025747:c3874534-3874220 [Escherichia sp. HH091_1A]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15039.43 Da        Isoelectric Point: 4.3697

>AT40763 WP_000560267.1 NZ_AP025747:c3874223-3873807 [Escherichia sp. HH091_1A]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQDMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT40763 NZ_AP025747:c3874223-3873807 [Escherichia sp. HH091_1A]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTTTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCTGAATTTAATGCCATGGCTCAAGACATGCCTGGCGGT
ATAGCTGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References