Detailed information of TA system TA040761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3427683..3428410
• Replicon: chromosome
• Accession: NZ_AP025747
• Organism: Escherichia sp. HH091_1A strain HH-P024

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0L6ZX69
• Locus tag: QUF14_RS17325
• Protein ID: WP_000547560.1
• Coordinates: 3428099..3428410 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUF14_RS17320
• Protein ID: WP_000126290.1
• Coordinates: 3427683..3428102 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF14_RS17300 (3422731)	3422731..3423258	-	528	WP_001078777.1	electron transport protein HydN	-
QUF14_RS17305 (3423408)	3423408..3424418	-	1011	WP_032193944.1	DNA-binding transcriptional regulator AscG	-
QUF14_RS17310 (3424678)	3424678..3426135	+	1458	WP_001107839.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
QUF14_RS17315 (3426144)	3426144..3427568	+	1425	WP_000110369.1	6-phospho-beta-glucosidase AscB	-
QUF14_RS17320 (3427683)	3427683..3428102	-	420	WP_000126290.1	helix-turn-helix domain-containing protein	Antitoxin
QUF14_RS17325 (3428099)	3428099..3428410	-	312	WP_000547560.1	type II toxin-antitoxin system HigB family toxin	Toxin
QUF14_RS17330 (3428578)	3428578..3429048	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
QUF14_RS17335 (3429041)	3429041..3429451	-	411	WP_001291923.1	formate hydrogenlyase assembly protein HycH	-
QUF14_RS17340 (3429448)	3429448..3430215	-	768	WP_000067390.1	formate hydrogenlyase subunit HycG	-
QUF14_RS17345 (3430215)	3430215..3430757	-	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
QUF14_RS17350 (3430767)	3430767..3432476	-	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12423.12 Da
• Isoelectric Point: 9.7106

>T40761 WP_000547560.1 NZ_AP025747:c3428410-3428099 [Escherichia sp. HH091_1A]
MHIISKAPFEESARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T40761 NZ_AP025747:c3428410-3428099 [Escherichia sp. HH091_1A]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAATAAATGGTACGTTCTGGATGTAGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCATGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15453.48 Da
• Isoelectric Point: 4.5628

>AT40761 WP_000126290.1 NZ_AP025747:c3428102-3427683 [Escherichia sp. HH091_1A]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNHEKFAEFDKAVAAMPI
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT40761 NZ_AP025747:c3428102-3427683 [Escherichia sp. HH091_1A]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGTATTGCAGAATATGAAAACAACCACGAAAAGTTTGCAGAGTTCGATAAAGCCGTTGCAGCAATGCCGATT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAAAGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0L6ZX69