Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1875665..1876227 Replicon chromosome
Accession NZ_AP025747
Organism Escherichia sp. HH091_1A strain HH-P024

Toxin (Protein)


Gene name higB Uniprot ID E9XKD5
Locus tag QUF14_RS09370 Protein ID WP_000605673.1
Coordinates 1875949..1876227 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag QUF14_RS09365 Protein ID WP_000781370.1
Coordinates 1875665..1875949 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUF14_RS09350 (1871024) 1871024..1874071 + 3048 WP_145960282.1 formate dehydrogenase-N subunit alpha -
QUF14_RS09355 (1874084) 1874084..1874968 + 885 WP_001240580.1 formate dehydrogenase N subunit beta -
QUF14_RS09360 (1874961) 1874961..1875614 + 654 WP_000045648.1 formate dehydrogenase-N subunit gamma -
QUF14_RS09365 (1875665) 1875665..1875949 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
QUF14_RS09370 (1875949) 1875949..1876227 - 279 WP_000605673.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QUF14_RS09375 (1876413) 1876413..1877423 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
QUF14_RS09380 (1877558) 1877558..1879255 - 1698 WP_000433464.1 malate dehydrogenase -
QUF14_RS09385 (1879412) 1879412..1879549 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
QUF14_RS09390 (1879651) 1879651..1879866 - 216 WP_000495766.1 biofilm-dependent modulation protein -
QUF14_RS09395 (1880210) 1880210..1880641 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10535.13 Da        Isoelectric Point: 7.3562

>T40752 WP_000605673.1 NZ_AP025747:c1876227-1875949 [Escherichia sp. HH091_1A]
MIMNFRHKGLRDLFLLGKTSGVIPTQIKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T40752 NZ_AP025747:c1876227-1875949 [Escherichia sp. HH091_1A]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAAT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGTGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT40752 WP_000781370.1 NZ_AP025747:c1875949-1875665 [Escherichia sp. HH091_1A]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT40752 NZ_AP025747:c1875949-1875665 [Escherichia sp. HH091_1A]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAGCTGAACGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCCGCGTTGACGCCAGAAATGGCGA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTACAAAACGCCTGGAGTCTGGCAGAGGCAGAA
AAAACGGTAGATGTGTCGCGACTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7ADF6

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References