Detailed information of TA system TA040728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 59711..60282
• Replicon: plasmid pla1
• Accession: NZ_AP025742
• Organism: Escherichia sp. 10290

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B0VD50
• Locus tag: QUE74_RS27650
• Protein ID: WP_024166187.1
• Coordinates: 60001..60282 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: QUE74_RS27645
• Protein ID: WP_001081196.1
• Coordinates: 59711..59992 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE74_RS27620 (56101)	56101..56377	+	277	Protein_63	transposase	-
QUE74_RS27625 (56645)	56645..57649	-	1005	WP_001179711.1	IS110 family transposase	-
QUE74_RS27630 (57698)	57698..58656	+	959	Protein_65	IS3 family transposase	-
QUE74_RS27635 (58809)	58809..59462	+	654	Protein_66	helix-turn-helix domain-containing protein	-
QUE74_RS27640 (59463)	59463..59546	-	84	Protein_67	hypothetical protein	-
QUE74_RS27645 (59711)	59711..59992	-	282	WP_001081196.1	HigA family addiction module antitoxin	Antitoxin
QUE74_RS27650 (60001)	60001..60282	-	282	WP_024166187.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUE74_RS27655 (60447)	60447..61690	-	1244	Protein_70	group II intron reverse transcriptase/maturase	-
QUE74_RS27660 (62089)	62089..62267	+	179	Protein_71	hypothetical protein	-
QUE74_RS27665 (62271)	62271..62502	-	232	Protein_72	arylsulfatase regulator	-
QUE74_RS27670 (62525)	62525..63103	-	579	WP_000136193.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyD / hlyD / hlyB / hlyB / hlyA / hlyA / hlyC / estIa / faeJ / faeI / faeH / faeF / faeE / faeD / faeC / espP	1..224339	224339
	-	inside	IScluster/Tn	-	-	33495..69706	36211

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-64)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10616.24 Da
• Isoelectric Point: 10.4126

>T40728 WP_024166187.1 NZ_AP025742:c60282-60001 [Escherichia sp. 10290]
MIKSFRHKGLEHFFKTGSTAGIQAKHAVKLRIQLTALNMAKRPDDMSAPGWKLHPLKGADLKGHWAISVNGNWRLTFRFE
GEDAILVDYQDYH

Nucleotide

• Length: 282 bp

>T40728 NZ_AP025742:c60282-60001 [Escherichia sp. 10290]
TTGATTAAGTCATTTCGCCATAAAGGGCTTGAACACTTTTTCAAGACAGGTTCAACCGCAGGCATTCAGGCCAAACATGC
GGTTAAGCTACGCATTCAATTAACCGCCCTTAATATGGCTAAACGCCCAGACGATATGTCTGCACCAGGCTGGAAATTAC
ACCCTCTGAAGGGAGCTGATCTTAAAGGCCATTGGGCTATATCTGTAAACGGTAACTGGCGACTTACCTTCCGATTTGAA
GGTGAAGACGCAATTCTGGTTGACTATCAGGATTATCATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10191.65 Da
• Isoelectric Point: 7.4026

>AT40728 WP_001081196.1 NZ_AP025742:c59992-59711 [Escherichia sp. 10290]
MNRMHNPAHPGAVLREYLGEMSVTDAATALGITRTALSRILNEKAGISADMALRLESALGTSAEMWTGMQSQYELWVASQ
TARPNIRPLFHHA

Nucleotide

• Length: 282 bp

>AT40728 NZ_AP025742:c59992-59711 [Escherichia sp. 10290]
ATGAATCGTATGCATAATCCCGCGCACCCAGGCGCGGTTCTTCGTGAATATCTGGGAGAAATGTCAGTCACAGACGCTGC
CACCGCATTAGGCATTACTCGCACGGCGCTTTCCCGGATTCTGAATGAAAAAGCTGGTATCTCTGCGGATATGGCGCTCC
GCCTGGAATCCGCACTCGGAACTAGCGCCGAAATGTGGACTGGTATGCAATCTCAGTATGAGCTTTGGGTTGCTTCGCAA
ACCGCTCGCCCAAACATTCGCCCATTATTTCACCATGCCTGA

Structures

Toxin

• AlphaFold DB: A0A0B0VD50