Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3956499..3957226 Replicon chromosome
Accession NZ_AP025741
Organism Escherichia sp. 10290

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag QUE74_RS19990 Protein ID WP_000550189.1
Coordinates 3956912..3957226 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag QUE74_RS19985 Protein ID WP_000560267.1
Coordinates 3956499..3956915 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE74_RS19975 (3951761) 3951761..3954112 + 2352 WP_000695509.1 alpha-glucosidase -
QUE74_RS19980 (3954436) 3954436..3956454 + 2019 WP_000121495.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
QUE74_RS19985 (3956499) 3956499..3956915 - 417 WP_000560267.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
QUE74_RS19990 (3956912) 3956912..3957226 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
QUE74_RS19995 (3957454) 3957454..3958590 - 1137 WP_000018674.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
QUE74_RS20000 (3958675) 3958675..3959178 + 504 WP_001295542.1 M48 family metallopeptidase -
QUE74_RS20005 (3959255) 3959255..3959947 + 693 WP_000942563.1 vancomycin high temperature exclusion protein -
QUE74_RS20010 (3960026) 3960026..3961012 + 987 WP_001360983.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T40722 WP_000550189.1 NZ_AP025741:c3957226-3956912 [Escherichia sp. 10290]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T40722 NZ_AP025741:c3957226-3956912 [Escherichia sp. 10290]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15039.43 Da        Isoelectric Point: 4.3697

>AT40722 WP_000560267.1 NZ_AP025741:c3956915-3956499 [Escherichia sp. 10290]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQDMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT40722 NZ_AP025741:c3956915-3956499 [Escherichia sp. 10290]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTTTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCTGAATTTAATGCCATGGCTCAAGACATGCCTGGCGGT
ATAGCTGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References