Detailed information of TA system TA040720

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3507070..3507797
• Replicon: chromosome
• Accession: NZ_AP025741
• Organism: Escherichia sp. 10290

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0L6ZX69
• Locus tag: QUE74_RS17815
• Protein ID: WP_000547560.1
• Coordinates: 3507486..3507797 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: QUE74_RS17810
• Protein ID: WP_000126290.1
• Coordinates: 3507070..3507489 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE74_RS17790 (3502118)	3502118..3502645	-	528	WP_001078777.1	electron transport protein HydN	-
QUE74_RS17795 (3502795)	3502795..3503805	-	1011	WP_032193944.1	DNA-binding transcriptional regulator AscG	-
QUE74_RS17800 (3504065)	3504065..3505522	+	1458	WP_001107903.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
QUE74_RS17805 (3505531)	3505531..3506955	+	1425	WP_000110369.1	6-phospho-beta-glucosidase AscB	-
QUE74_RS17810 (3507070)	3507070..3507489	-	420	WP_000126290.1	helix-turn-helix domain-containing protein	Antitoxin
QUE74_RS17815 (3507486)	3507486..3507797	-	312	WP_000547560.1	type II toxin-antitoxin system HigB family toxin	Toxin
QUE74_RS17820 (3507965)	3507965..3508435	-	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
QUE74_RS17825 (3508428)	3508428..3508838	-	411	WP_001291923.1	formate hydrogenlyase assembly protein HycH	-
QUE74_RS17830 (3508835)	3508835..3509602	-	768	WP_000067390.1	formate hydrogenlyase subunit HycG	-
QUE74_RS17835 (3509602)	3509602..3510144	-	543	WP_000493798.1	formate hydrogenlyase subunit HycF	-
QUE74_RS17840 (3510154)	3510154..3511863	-	1710	WP_001288122.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12423.12 Da
• Isoelectric Point: 9.7106

>T40720 WP_000547560.1 NZ_AP025741:c3507797-3507486 [Escherichia sp. 10290]
MHIISKAPFEESARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T40720 NZ_AP025741:c3507797-3507486 [Escherichia sp. 10290]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAATAAATGGTACGTTCTGGATGTAGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCATGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15453.48 Da
• Isoelectric Point: 4.5628

>AT40720 WP_000126290.1 NZ_AP025741:c3507489-3507070 [Escherichia sp. 10290]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNHEKFAEFDKAVAAMPI
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT40720 NZ_AP025741:c3507489-3507070 [Escherichia sp. 10290]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGTATTGCAGAATATGAAAACAACCACGAAAAGTTTGCAGAGTTCGATAAAGCCGTTGCAGCAATGCCGATT
GGCGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAAAGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0L6ZX69