Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1943518..1944080 Replicon chromosome
Accession NZ_AP025741
Organism Escherichia sp. 10290

Toxin (Protein)


Gene name higB Uniprot ID E9XKD5
Locus tag QUE74_RS09800 Protein ID WP_000605673.1
Coordinates 1943802..1944080 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag QUE74_RS09795 Protein ID WP_000781370.1
Coordinates 1943518..1943802 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE74_RS09780 (1938700) 1938700..1941747 + 3048 WP_015953395.1 formate dehydrogenase-N subunit alpha -
QUE74_RS09785 (1941760) 1941760..1942644 + 885 WP_001240580.1 formate dehydrogenase N subunit beta -
QUE74_RS09790 (1942637) 1942637..1943290 + 654 WP_000045648.1 formate dehydrogenase-N subunit gamma -
QUE74_RS09795 (1943518) 1943518..1943802 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
QUE74_RS09800 (1943802) 1943802..1944080 - 279 WP_000605673.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QUE74_RS09805 (1944266) 1944266..1945276 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
QUE74_RS09810 (1945411) 1945411..1947108 - 1698 WP_000433464.1 malate dehydrogenase -
QUE74_RS09815 (1947265) 1947265..1947402 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
QUE74_RS09820 (1947504) 1947504..1947719 - 216 WP_000495766.1 biofilm-dependent modulation protein -
QUE74_RS09825 (1948063) 1948063..1948494 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10535.13 Da        Isoelectric Point: 7.3562

>T40709 WP_000605673.1 NZ_AP025741:c1944080-1943802 [Escherichia sp. 10290]
MIMNFRHKGLRDLFLLGKTSGVIPTQIKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T40709 NZ_AP025741:c1944080-1943802 [Escherichia sp. 10290]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAAT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGTGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT40709 WP_000781370.1 NZ_AP025741:c1943802-1943518 [Escherichia sp. 10290]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT40709 NZ_AP025741:c1943802-1943518 [Escherichia sp. 10290]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAGCTGAACGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCCGCGTTGACGCCAGAAATGGCGA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTACAAAACGCCTGGAGTCTGGCAGAGGCAGAA
AAAACGGTAGATGTGTCGCGATTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7ADF6

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References