Detailed information of TA system TA040653

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4008652..4009328
• Replicon: chromosome
• Accession: NZ_AP025692
• Organism: Streptomyces sp. PLM4

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: MTP06_RS17480
• Protein ID: WP_018471256.1
• Coordinates: 4008652..4009014 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MTP06_RS17485
• Protein ID: WP_018471255.1
• Coordinates: 4009011..4009328 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTP06_RS17445 (MTP06_35130)	4003714..4004784	-	1071	WP_010643524.1	ATP-binding protein/SpoIIE family protein phosphatase	-
MTP06_RS17450 (MTP06_35140)	4004781..4005197	-	417	WP_003949884.1	ATP-binding protein	-
MTP06_RS17455 (MTP06_35150)	4005197..4005577	-	381	WP_003949885.1	STAS domain-containing protein	-
MTP06_RS17460 (MTP06_35160)	4005574..4006428	-	855	WP_037844512.1	STAS domain-containing protein	-
MTP06_RS17465 (MTP06_35170)	4006732..4006974	+	243	WP_037844511.1	hypothetical protein	-
MTP06_RS17470 (MTP06_35180)	4007065..4007949	-	885	WP_234364568.1	LPXTG cell wall anchor domain-containing protein	-
MTP06_RS17475 (MTP06_35190)	4007868..4008653	-	786	WP_248495504.1	hypothetical protein	-
MTP06_RS17480 (MTP06_35200)	4008652..4009014	+	363	WP_018471256.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MTP06_RS17485 (MTP06_35210)	4009011..4009328	+	318	WP_018471255.1	helix-turn-helix transcriptional regulator	Antitoxin
MTP06_RS17490 (MTP06_35220)	4009407..4010189	-	783	WP_003949890.1	ABC transporter permease	-
MTP06_RS17495 (MTP06_35230)	4010186..4011160	-	975	WP_248496813.1	ATP-binding cassette domain-containing protein	-
MTP06_RS17500 (MTP06_35240)	4011258..4012127	-	870	WP_129805594.1	DUF4097 family beta strand repeat-containing protein	-
MTP06_RS17505 (MTP06_35250)	4012229..4012759	-	531	WP_003949893.1	toxin-antitoxin system HicB family antitoxin	-
MTP06_RS17510 (MTP06_35260)	4012965..4014026	-	1062	WP_030770447.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-87)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13502.36 Da
• Isoelectric Point: 4.6168

>T40653 WP_018471256.1 NZ_AP025692:4008652-4009014 [Streptomyces sp. PLM4]
MDGEWQIFLVDEVRVWLASLDGAAHARVVQALDVLAEEGPALGRPLVDTLRGSAVANLKELRPGTVRILFAFDPWRSSIL
LVAGDKSGQWTEWYQEAIPLAEQRYALYLKEREREEGGRP

Nucleotide

• Length: 363 bp

>T40653 NZ_AP025692:4008652-4009014 [Streptomyces sp. PLM4]
ATGGACGGGGAATGGCAGATCTTCCTGGTTGACGAGGTGCGTGTTTGGCTCGCGTCCCTCGACGGGGCCGCGCACGCACG
AGTGGTGCAGGCGCTGGACGTGCTGGCCGAGGAAGGGCCTGCTCTCGGAAGACCGCTTGTGGACACCTTGCGTGGTTCAG
CGGTGGCCAATTTGAAAGAGCTCCGGCCCGGCACGGTCAGAATTCTGTTCGCCTTCGATCCGTGGCGTTCCAGCATCTTG
CTGGTGGCAGGTGACAAGTCCGGGCAGTGGACTGAGTGGTACCAGGAGGCGATCCCCCTGGCCGAGCAGCGCTACGCGCT
TTACTTGAAGGAACGTGAGCGGGAAGAGGGAGGACGACCATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11558.05 Da
• Isoelectric Point: 9.8236

>AT40653 WP_018471255.1 NZ_AP025692:4009011-4009328 [Streptomyces sp. PLM4]
MTSYTRWQDIRPEHVARAGGEEAVRAGKEELLAQVTGRKLAELRRARGLTQQEVADRMGVSKGRVSQIERGHLSGQEVLA
RFATALGGRLHQAIYFDDGDIATIA

Nucleotide

• Length: 318 bp

>AT40653 NZ_AP025692:4009011-4009328 [Streptomyces sp. PLM4]
ATGACGAGTTACACCCGTTGGCAGGACATTCGTCCCGAGCATGTCGCCCGCGCAGGGGGTGAGGAAGCCGTACGCGCGGG
TAAGGAGGAGCTGCTCGCGCAGGTCACCGGGAGGAAGCTCGCGGAGCTGCGTCGCGCCCGAGGGCTGACCCAGCAGGAGG
TGGCGGACCGGATGGGGGTCAGCAAGGGGCGGGTCTCCCAGATCGAGCGGGGGCACCTTTCGGGACAGGAAGTGCTGGCC
CGATTCGCGACCGCTCTCGGAGGCCGTCTCCATCAGGCGATCTACTTCGACGACGGCGACATCGCCACCATCGCCTGA