Detailed information of TA system TA040644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2457393..2457910
• Replicon: chromosome
• Accession: NZ_AP025683
• Organism: Staphylococcus aureus strain JP025

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A170NWK0
• Locus tag: SAJP025_RS12145
• Protein ID: WP_000251928.1
• Coordinates: 2457393..2457659 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A166LMC5
• Locus tag: SAJP025_RS12150
• Protein ID: WP_000584497.1
• Coordinates: 2457659..2457910 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SAJP025_RS12120 (2452600)	2452600..2453136	-	537	WP_000024292.1	GNAT family N-acetyltransferase	-
SAJP025_RS12125 (2453604)	2453604..2454428	-	825	WP_000594640.1	formate/nitrite transporter family protein	-
SAJP025_RS12130 (2454631)	2454631..2454813	-	183	WP_000079140.1	hypothetical protein	-
SAJP025_RS12135 (2454900)	2454900..2455367	-	468	WP_000153340.1	SRPBCC domain-containing protein	-
SAJP025_RS12140 (2455553)	2455553..2457100	-	1548	WP_000727771.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
SAJP025_RS12145 (2457393)	2457393..2457659	-	267	WP_000251928.1	Txe/YoeB family addiction module toxin	Toxin
SAJP025_RS12150 (2457659)	2457659..2457910	-	252	WP_000584497.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
SAJP025_RS12155 (2458074)	2458074..2458174	-	101	Protein_2353	hypothetical protein	-
SAJP025_RS12160 (2458181)	2458181..2458780	-	600	WP_000162804.1	DsbA family protein	-
SAJP025_RS12165 (2458799)	2458799..2459161	-	363	WP_000819843.1	DUF4467 domain-containing protein	-
SAJP025_RS12170 (2459416)	2459416..2460666	+	1251	WP_001012237.1	FemA/FemB family glycyltransferase FmhA	-
SAJP025_RS12175 (2460763)	2460763..2461494	-	732	WP_000615467.1	amino acid ABC transporter ATP-binding protein	-
SAJP025_RS12180 (2461491)	2461491..2462210	-	720	WP_000479573.1	amino acid ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (8-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10465.06 Da
• Isoelectric Point: 10.1628

>T40644 WP_000251928.1 NZ_AP025683:c2457659-2457393 [Staphylococcus aureus]
MVRLNITFSPQAFEDYKYFQQNDKKMVKKINELIKSIDRNGALKGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T40644 NZ_AP025683:c2457659-2457393 [Staphylococcus aureus]
ATGGTTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTAATTAAAAGTATTGACAGAAATGGTGCGTTGAAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9401.54 Da
• Isoelectric Point: 4.3407

>AT40644 WP_000584497.1 NZ_AP025683:c2457910-2457659 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDIVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTVTKD
IDV

Nucleotide

• Length: 252 bp

>AT40644 NZ_AP025683:c2457910-2457659 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATAGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAATCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTGTAACGAAAGAT
ATAGATGTATAA

Structures

Toxin

• AlphaFold DB: A0A170NWK0

Antitoxin

• AlphaFold DB: A0A166LMC5