Detailed information of TA system TA040627

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4730309..4730911
• Replicon: chromosome
• Accession: NZ_AP025682
• Organism: Escherichia coli strain Rb-50

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: EcoliRb_RS22620
• Protein ID: WP_000897302.1
• Coordinates: 4730600..4730911 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EcoliRb_RS22615
• Protein ID: WP_000356397.1
• Coordinates: 4730309..4730599 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EcoliRb_RS22590 (4726382)	4726382..4727284	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
EcoliRb_RS22595 (4727281)	4727281..4727916	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
EcoliRb_RS22600 (4727913)	4727913..4728842	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
EcoliRb_RS22605 (4729058)	4729058..4729276	-	219	WP_001314326.1	CopG family transcriptional regulator	-
EcoliRb_RS22610 (4729672)	4729672..4729950	-	279	WP_001296612.1	hypothetical protein	-
EcoliRb_RS22615 (4730309)	4730309..4730599	-	291	WP_000356397.1	NadS family protein	Antitoxin
EcoliRb_RS22620 (4730600)	4730600..4730911	-	312	WP_000897302.1	hypothetical protein	Toxin
EcoliRb_RS22625 (4731140)	4731140..4732048	+	909	WP_001331553.1	alpha/beta hydrolase	-
EcoliRb_RS22630 (4732112)	4732112..4733053	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
EcoliRb_RS22635 (4733098)	4733098..4733535	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
EcoliRb_RS22640 (4733532)	4733532..4734404	-	873	WP_000920762.1	virulence factor BrkB family protein	-
EcoliRb_RS22645 (4734398)	4734398..4734997	-	600	WP_001298594.1	glucose-1-phosphatase	-
EcoliRb_RS22650 (4735096)	4735096..4735881	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T40627 WP_000897302.1 NZ_AP025682:c4730911-4730600 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T40627 NZ_AP025682:c4730911-4730600 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT40627 WP_000356397.1 NZ_AP025682:c4730599-4730309 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT40627 NZ_AP025682:c4730599-4730309 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416