Detailed information of TA system TA040621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3727685..3728379
• Replicon: chromosome
• Accession: NZ_AP025682
• Organism: Escherichia coli strain Rb-50

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: EcoliRb_RS18005
• Protein ID: WP_137502061.1
• Coordinates: 3727685..3728083 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: EcoliRb_RS18010
• Protein ID: WP_000554755.1
• Coordinates: 3728086..3728379 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EcoliRb_RS17980 (3723050)	3723050..3723508	-	459	WP_001291991.1	xanthine phosphoribosyltransferase	-
EcoliRb_RS17985 (3723769)	3723769..3725226	+	1458	WP_105466882.1	cytosol nonspecific dipeptidase	-
EcoliRb_RS17990 (3725283)	3725283..3725897	-	615	WP_000602135.1	peptide chain release factor H	-
EcoliRb_RS17995 (3725894)	3725894..3727033	-	1140	WP_000521558.1	RNA ligase RtcB family protein	-
EcoliRb_RS18000 (3727223)	3727223..3727675	-	453	WP_001059897.1	GNAT family N-acetyltransferase	-
EcoliRb_RS18005 (3727685)	3727685..3728083	-	399	WP_137502061.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
EcoliRb_RS18010 (3728086)	3728086..3728379	-	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
EcoliRb_RS18015 (3728431)	3728431..3729486	-	1056	WP_001226166.1	DNA polymerase IV	-
EcoliRb_RS18020 (3729557)	3729557..3730480	-	924	WP_001232566.1	putative lateral flagellar export/assembly protein LafU	-
EcoliRb_RS18025 (3730483)	3730483..3731346	-	864	WP_001169519.1	flagellar motor stator protein MotA	-
EcoliRb_RS18030 (3731359)	3731359..3732078	-	720	WP_137501514.1	FliA/WhiG family RNA polymerase sigma factor	-
EcoliRb_RS18035 (3732098)	3732098..3732565	-	468	WP_000725264.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15399.82 Da
• Isoelectric Point: 8.9511

>T40621 WP_137502061.1 NZ_AP025682:c3728083-3727685 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T40621 NZ_AP025682:c3728083-3727685 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT40621 WP_000554755.1 NZ_AP025682:c3728379-3728086 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT40621 NZ_AP025682:c3728379-3728086 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A829G9H5