Detailed information of TA system TA040606

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 616142..616941
• Replicon: chromosome
• Accession: NZ_AP025682
• Organism: Escherichia coli strain Rb-50

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: EcoliRb_RS03005
• Protein ID: WP_000347273.1
• Coordinates: 616142..616606 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: EcoliRb_RS03010
• Protein ID: WP_001308975.1
• Coordinates: 616606..616941 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EcoliRb_RS02975 (611143)	611143..611577	-	435	WP_115439031.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
EcoliRb_RS02980 (611595)	611595..612473	-	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
EcoliRb_RS02985 (612463)	612463..613242	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
EcoliRb_RS02990 (613253)	613253..613726	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
EcoliRb_RS02995 (613749)	613749..615029	-	1281	WP_244331656.1	tagatose-bisphosphate aldolase subunit KbaZ	-
EcoliRb_RS03000 (615278)	615278..616087	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
EcoliRb_RS03005 (616142)	616142..616606	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
EcoliRb_RS03010 (616606)	616606..616941	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
EcoliRb_RS03015 (617090)	617090..618661	-	1572	WP_001273756.1	galactarate dehydratase	-
EcoliRb_RS03020 (619036)	619036..620370	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
EcoliRb_RS03025 (620386)	620386..621156	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T40606 WP_000347273.1 NZ_AP025682:c616606-616142 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T40606 NZ_AP025682:c616606-616142 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT40606 WP_001308975.1 NZ_AP025682:c616941-616606 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT40606 NZ_AP025682:c616941-616606 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08