Detailed information of TA system TA040604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4594018..4594620
• Replicon: chromosome
• Accession: NZ_AP025677
• Organism: Escherichia coli strain Rb-23

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3L4YH82
• Locus tag: MWM20_RS22220
• Protein ID: WP_000897303.1
• Coordinates: 4594309..4594620 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MWM20_RS22215
• Protein ID: WP_000356397.1
• Coordinates: 4594018..4594308 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWM20_RS22185 (4589323)	4589323..4590252	+	930	WP_000027695.1	formate dehydrogenase accessory protein FdhE	-
MWM20_RS22190 (4590434)	4590434..4590676	-	243	WP_001068516.1	CopG family transcriptional regulator	-
MWM20_RS22195 (4590966)	4590966..4591814	+	849	WP_001038650.1	hypothetical protein	-
MWM20_RS22200 (4591839)	4591839..4592579	+	741	WP_000608807.1	hypothetical protein	-
MWM20_RS22205 (4592764)	4592764..4592982	-	219	WP_001251289.1	CopG family transcriptional regulator	-
MWM20_RS22210 (4593381)	4593381..4593659	-	279	WP_001296612.1	hypothetical protein	-
MWM20_RS22215 (4594018)	4594018..4594308	-	291	WP_000356397.1	NadS family protein	Antitoxin
MWM20_RS22220 (4594309)	4594309..4594620	-	312	WP_000897303.1	hypothetical protein	Toxin
MWM20_RS22225 (4594849)	4594849..4595757	+	909	WP_001385591.1	alpha/beta hydrolase	-
MWM20_RS22230 (4595821)	4595821..4596762	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
MWM20_RS22235 (4596807)	4596807..4597244	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
MWM20_RS22240 (4597241)	4597241..4598113	-	873	WP_000920764.1	virulence factor BrkB family protein	-
MWM20_RS22245 (4598107)	4598107..4598706	-	600	WP_001296610.1	glucose-1-phosphatase	-
MWM20_RS22250 (4598805)	4598805..4599590	-	786	WP_000059675.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12302.33 Da
• Isoelectric Point: 10.0233

>T40604 WP_000897303.1 NZ_AP025677:c4594620-4594309 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGRLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T40604 NZ_AP025677:c4594620-4594309 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTAGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAGGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT40604 WP_000356397.1 NZ_AP025677:c4594308-4594018 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT40604 NZ_AP025677:c4594308-4594018 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A3L4YH82