Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4273913..4274640 Replicon chromosome
Accession NZ_AP025677
Organism Escherichia coli strain Rb-23

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag MWM20_RS20650 Protein ID WP_000550189.1
Coordinates 4274326..4274640 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag MWM20_RS20645 Protein ID WP_000560266.1
Coordinates 4273913..4274329 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWM20_RS20635 (4269273) 4269273..4271624 + 2352 WP_000695436.1 alpha-glucosidase -
MWM20_RS20640 (4271850) 4271850..4273868 + 2019 WP_244345529.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
MWM20_RS20645 (4273913) 4273913..4274329 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
MWM20_RS20650 (4274326) 4274326..4274640 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
MWM20_RS20655 (4274925) 4274925..4276061 - 1137 WP_000018705.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
MWM20_RS20660 (4276146) 4276146..4276649 + 504 WP_001295542.1 M48 family metallopeptidase -
MWM20_RS20665 (4276726) 4276726..4277418 + 693 WP_244345530.1 vancomycin high temperature exclusion protein -
MWM20_RS20670 (4277497) 4277497..4278483 + 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T40602 WP_000550189.1 NZ_AP025677:c4274640-4274326 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T40602 NZ_AP025677:c4274640-4274326 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCAGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT40602 WP_000560266.1 NZ_AP025677:c4274329-4273913 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT40602 NZ_AP025677:c4274329-4273913 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATTGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References