Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2562158..2562720 Replicon chromosome
Accession NZ_AP025677
Organism Escherichia coli strain Rb-23

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag MWM20_RS12615 Protein ID WP_000605675.1
Coordinates 2562442..2562720 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag MWM20_RS12610 Protein ID WP_000781370.1
Coordinates 2562158..2562442 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWM20_RS12595 (2557338) 2557338..2560385 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
MWM20_RS12600 (2560398) 2560398..2561282 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
MWM20_RS12605 (2561275) 2561275..2561928 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
MWM20_RS12610 (2562158) 2562158..2562442 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
MWM20_RS12615 (2562442) 2562442..2562720 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MWM20_RS12620 (2562906) 2562906..2563916 - 1011 WP_000642417.1 alcohol dehydrogenase AdhP -
MWM20_RS12625 (2564050) 2564050..2565747 - 1698 WP_000433461.1 malate dehydrogenase -
MWM20_RS12630 (2565903) 2565903..2566040 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
MWM20_RS12635 (2566142) 2566142..2566357 - 216 WP_000495774.1 biofilm-dependent modulation protein -
MWM20_RS12640 (2566701) 2566701..2567132 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T40590 WP_000605675.1 NZ_AP025677:c2562720-2562442 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T40590 NZ_AP025677:c2562720-2562442 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT40590 WP_000781370.1 NZ_AP025677:c2562442-2562158 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT40590 NZ_AP025677:c2562442-2562158 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References