Detailed information of TA system TA040579

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): HipBST/HipA(toxin)
• Location: 183147..184465
• Replicon: chromosome
• Accession: NZ_AP025677
• Organism: Escherichia coli strain Rb-23

Toxin (Protein)

• Gene name: HipT
• Uniprot ID: F4T5A3
• Locus tag: MWM20_RS00870
• Protein ID: WP_001262467.1
• Coordinates: 183458..184465 (+)
• Length: 336 a.a.

Antitoxin (Protein)

• Gene name: HipS
• Uniprot ID: F4T5A4
• Locus tag: MWM20_RS00865
• Protein ID: WP_001312177.1
• Coordinates: 183147..183458 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWM20_RS00835 (178434)	178434..178661	-	228	WP_000198576.1	hypothetical protein	-
MWM20_RS00840 (178679)	178679..179698	+	1020	WP_000938855.1	dipeptide ABC transporter permease DppB	-
MWM20_RS00845 (179708)	179708..180610	+	903	WP_000084677.1	dipeptide ABC transporter permease DppC	-
MWM20_RS00850 (180621)	180621..181604	+	984	WP_001196477.1	dipeptide ABC transporter ATP-binding protein	-
MWM20_RS00855 (181601)	181601..182614	+	1014	WP_000103555.1	dipeptide ABC transporter ATP-binding subunit DppF	-
MWM20_RS00860 (182839)	182839..183162	+	324	WP_000563102.1	helix-turn-helix transcriptional regulator	-
MWM20_RS00865 (183147)	183147..183458	+	312	WP_001312177.1	HipA N-terminal domain-containing protein	Antitoxin
MWM20_RS00870 (183458)	183458..184465	+	1008	WP_001262467.1	HipA domain-containing protein	Toxin
MWM20_RS00875 (184482)	184482..185753	-	1272	WP_001312176.1	amino acid permease	-
- (186115)	186115..186180	-	66	NuclAT_26	-	-
- (186115)	186115..186180	-	66	NuclAT_26	-	-
- (186115)	186115..186180	-	66	NuclAT_26	-	-
- (186115)	186115..186180	-	66	NuclAT_26	-	-
- (186115)	186115..186180	-	66	NuclAT_29	-	-
- (186115)	186115..186180	-	66	NuclAT_29	-	-
- (186115)	186115..186180	-	66	NuclAT_29	-	-
- (186115)	186115..186180	-	66	NuclAT_29	-	-
MWM20_RS00880 (186229)	186229..186336	+	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
- (186598)	186598..186655	-	58	NuclAT_24	-	-
- (186598)	186598..186655	-	58	NuclAT_24	-	-
- (186598)	186598..186655	-	58	NuclAT_24	-	-
- (186598)	186598..186655	-	58	NuclAT_24	-	-
- (186598)	186598..186655	-	58	NuclAT_27	-	-
- (186598)	186598..186655	-	58	NuclAT_27	-	-
- (186598)	186598..186655	-	58	NuclAT_27	-	-
- (186598)	186598..186655	-	58	NuclAT_27	-	-
MWM20_RS00885 (186712)	186712..186819	+	108	WP_000170748.1	type I toxin-antitoxin system toxin Ldr family protein	-
- (187081)	187081..187146	-	66	NuclAT_25	-	-
- (187081)	187081..187146	-	66	NuclAT_25	-	-
- (187081)	187081..187146	-	66	NuclAT_25	-	-
- (187081)	187081..187146	-	66	NuclAT_25	-	-
- (187081)	187081..187146	-	66	NuclAT_28	-	-
- (187081)	187081..187146	-	66	NuclAT_28	-	-
- (187081)	187081..187146	-	66	NuclAT_28	-	-
- (187081)	187081..187146	-	66	NuclAT_28	-	-
MWM20_RS00890 (187195)	187195..187302	+	108	WP_001295224.1	type I toxin-antitoxin system toxin Ldr family protein	-
MWM20_RS00895 (187388)	187388..189067	-	1680	WP_000191571.1	cellulose biosynthesis protein BcsG	-
MWM20_RS00900 (189064)	189064..189255	-	192	WP_000988308.1	cellulose biosynthesis protein BcsF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (56-302)

Antitoxin

• HipA, N-terminal subdomain 1 (6-98)

Sequences

Toxin

Protein

• Length: 336 a.a.
• Molecular weight: 38320.60 Da
• Isoelectric Point: 5.5965

>T40579 WP_001262467.1 NZ_AP025677:183458-184465 [Escherichia coli]
MANCRILLTPLNERDEQRGYSTQGLKRLSGTAKLTPRLGFTRTQFVQELPRQQKGMSISGYQPKLQLVLDEGEFRVVDHQ
GNFILKPSPADFPGLAENEHATMTLMSRLGFDVPVHGLLSFAPQSEEELEYAFVIRRYDRDNKGLPVHQEQLDGAMQIMD
KYGKTGNDNEQYVSYETLARFLVAHVNDNIAFKIDLFRRIVYAWLLGNNDMHLRNFGLVYSDGLTPALAPVYDFVSVAPY
PEYFHSNYLALPLLTREEGGRELAPGFHSDYGEYIGQDFLLLGESMGLAPRLLEKLFQDIRKENAIVMETYEQSFMTQDH
IQAVLQCYRHRLGLL

Nucleotide

• Length: 1008 bp

>T40579 NZ_AP025677:183458-184465 [Escherichia coli]
ATGGCGAATTGTCGTATTCTGTTAACGCCGTTGAATGAACGTGACGAACAGCGCGGATATTCGACACAGGGGTTGAAGCG
ACTGAGCGGCACTGCAAAATTGACCCCCAGGCTGGGGTTTACGCGAACTCAGTTTGTGCAGGAACTTCCGCGTCAACAAA
AAGGGATGAGTATTTCCGGTTACCAGCCCAAATTGCAATTAGTGCTGGATGAAGGAGAGTTCCGCGTCGTAGATCATCAG
GGAAATTTTATCCTTAAGCCCTCTCCAGCCGACTTTCCCGGTTTGGCCGAAAATGAACACGCAACAATGACATTGATGTC
TCGTTTGGGATTTGACGTACCTGTGCATGGCCTGTTGAGTTTTGCACCACAATCAGAGGAAGAGCTTGAATACGCATTTG
TTATCCGGCGCTATGACCGGGATAACAAGGGGCTGCCAGTGCATCAGGAACAGCTTGATGGCGCGATGCAGATTATGGAT
AAATATGGCAAAACGGGGAATGATAACGAACAATATGTCAGCTATGAAACGCTGGCCCGATTTTTAGTTGCCCATGTTAA
CGACAACATCGCATTTAAAATCGATCTCTTTCGTCGCATTGTTTACGCCTGGTTGCTGGGTAATAACGACATGCATTTAC
GAAACTTTGGCTTAGTGTACTCCGATGGTTTGACGCCAGCGTTAGCGCCGGTGTATGACTTTGTTTCTGTCGCTCCCTAT
CCGGAATATTTTCACTCAAACTATCTGGCGCTGCCGTTATTGACGCGAGAAGAAGGCGGTCGGGAGCTGGCTCCTGGATT
TCACAGTGATTATGGCGAGTATATCGGGCAGGACTTTTTGCTGTTGGGTGAATCAATGGGGTTAGCGCCCAGATTGCTGG
AAAAACTCTTTCAGGATATACGTAAAGAAAATGCCATTGTTATGGAAACGTATGAGCAGTCCTTTATGACTCAGGATCAT
ATCCAGGCTGTGCTGCAATGCTATCGACATCGTCTGGGGCTGCTGTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11821.47 Da
• Isoelectric Point: 6.0609

>AT40579 WP_001312177.1 NZ_AP025677:183147-183458 [Escherichia coli]
MHRRVKVLLYGQVVGELSQNDSGFLFQYAPDYHGPAISISLPVAQRQFPSEMLHPYFASLAPEGWLRQRYSQIQHRDEND
LLGMLIDNGKNLLGAIQILPWEE

Nucleotide

• Length: 312 bp

>AT40579 NZ_AP025677:183147-183458 [Escherichia coli]
ATGCATCGGCGAGTGAAAGTGTTGCTCTATGGTCAGGTAGTGGGTGAACTCTCTCAGAACGACAGTGGTTTTTTGTTCCA
GTATGCCCCGGATTACCACGGACCGGCAATCTCAATCAGCCTGCCCGTCGCACAGCGTCAGTTTCCCAGTGAGATGTTGC
ATCCTTATTTCGCCTCACTGGCGCCAGAAGGATGGCTGCGTCAACGCTACAGCCAGATACAGCATCGTGACGAAAACGAT
CTGCTGGGAATGCTTATTGATAACGGCAAGAATCTGCTGGGGGCCATCCAGATTTTACCTTGGGAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A3K2ZTB5

Antitoxin

• AlphaFold DB: A0A3L3HKE2