Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) HipBST/HipA(toxin)
Location 183147..184465 Replicon chromosome
Accession NZ_AP025677
Organism Escherichia coli strain Rb-23

Toxin (Protein)


Gene name HipT Uniprot ID F4T5A3
Locus tag MWM20_RS00870 Protein ID WP_001262467.1
Coordinates 183458..184465 (+) Length 336 a.a.

Antitoxin (Protein)


Gene name HipS Uniprot ID F4T5A4
Locus tag MWM20_RS00865 Protein ID WP_001312177.1
Coordinates 183147..183458 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWM20_RS00835 (178434) 178434..178661 - 228 WP_000198576.1 hypothetical protein -
MWM20_RS00840 (178679) 178679..179698 + 1020 WP_000938855.1 dipeptide ABC transporter permease DppB -
MWM20_RS00845 (179708) 179708..180610 + 903 WP_000084677.1 dipeptide ABC transporter permease DppC -
MWM20_RS00850 (180621) 180621..181604 + 984 WP_001196477.1 dipeptide ABC transporter ATP-binding protein -
MWM20_RS00855 (181601) 181601..182614 + 1014 WP_000103555.1 dipeptide ABC transporter ATP-binding subunit DppF -
MWM20_RS00860 (182839) 182839..183162 + 324 WP_000563102.1 helix-turn-helix transcriptional regulator -
MWM20_RS00865 (183147) 183147..183458 + 312 WP_001312177.1 HipA N-terminal domain-containing protein Antitoxin
MWM20_RS00870 (183458) 183458..184465 + 1008 WP_001262467.1 HipA domain-containing protein Toxin
MWM20_RS00875 (184482) 184482..185753 - 1272 WP_001312176.1 amino acid permease -
- (186115) 186115..186180 - 66 NuclAT_26 - -
- (186115) 186115..186180 - 66 NuclAT_26 - -
- (186115) 186115..186180 - 66 NuclAT_26 - -
- (186115) 186115..186180 - 66 NuclAT_26 - -
- (186115) 186115..186180 - 66 NuclAT_29 - -
- (186115) 186115..186180 - 66 NuclAT_29 - -
- (186115) 186115..186180 - 66 NuclAT_29 - -
- (186115) 186115..186180 - 66 NuclAT_29 - -
MWM20_RS00880 (186229) 186229..186336 + 108 WP_001295224.1 type I toxin-antitoxin system toxin Ldr family protein -
- (186598) 186598..186655 - 58 NuclAT_24 - -
- (186598) 186598..186655 - 58 NuclAT_24 - -
- (186598) 186598..186655 - 58 NuclAT_24 - -
- (186598) 186598..186655 - 58 NuclAT_24 - -
- (186598) 186598..186655 - 58 NuclAT_27 - -
- (186598) 186598..186655 - 58 NuclAT_27 - -
- (186598) 186598..186655 - 58 NuclAT_27 - -
- (186598) 186598..186655 - 58 NuclAT_27 - -
MWM20_RS00885 (186712) 186712..186819 + 108 WP_000170748.1 type I toxin-antitoxin system toxin Ldr family protein -
- (187081) 187081..187146 - 66 NuclAT_25 - -
- (187081) 187081..187146 - 66 NuclAT_25 - -
- (187081) 187081..187146 - 66 NuclAT_25 - -
- (187081) 187081..187146 - 66 NuclAT_25 - -
- (187081) 187081..187146 - 66 NuclAT_28 - -
- (187081) 187081..187146 - 66 NuclAT_28 - -
- (187081) 187081..187146 - 66 NuclAT_28 - -
- (187081) 187081..187146 - 66 NuclAT_28 - -
MWM20_RS00890 (187195) 187195..187302 + 108 WP_001295224.1 type I toxin-antitoxin system toxin Ldr family protein -
MWM20_RS00895 (187388) 187388..189067 - 1680 WP_000191571.1 cellulose biosynthesis protein BcsG -
MWM20_RS00900 (189064) 189064..189255 - 192 WP_000988308.1 cellulose biosynthesis protein BcsF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(56-302)

Antitoxin

HipA, N-terminal subdomain 1

(6-98)


Sequences

Toxin        


Download         Length: 336 a.a.        Molecular weight: 38320.60 Da        Isoelectric Point: 5.5965

>T40579 WP_001262467.1 NZ_AP025677:183458-184465 [Escherichia coli]
MANCRILLTPLNERDEQRGYSTQGLKRLSGTAKLTPRLGFTRTQFVQELPRQQKGMSISGYQPKLQLVLDEGEFRVVDHQ
GNFILKPSPADFPGLAENEHATMTLMSRLGFDVPVHGLLSFAPQSEEELEYAFVIRRYDRDNKGLPVHQEQLDGAMQIMD
KYGKTGNDNEQYVSYETLARFLVAHVNDNIAFKIDLFRRIVYAWLLGNNDMHLRNFGLVYSDGLTPALAPVYDFVSVAPY
PEYFHSNYLALPLLTREEGGRELAPGFHSDYGEYIGQDFLLLGESMGLAPRLLEKLFQDIRKENAIVMETYEQSFMTQDH
IQAVLQCYRHRLGLL

Download         Length: 1008 bp

>T40579 NZ_AP025677:183458-184465 [Escherichia coli]
ATGGCGAATTGTCGTATTCTGTTAACGCCGTTGAATGAACGTGACGAACAGCGCGGATATTCGACACAGGGGTTGAAGCG
ACTGAGCGGCACTGCAAAATTGACCCCCAGGCTGGGGTTTACGCGAACTCAGTTTGTGCAGGAACTTCCGCGTCAACAAA
AAGGGATGAGTATTTCCGGTTACCAGCCCAAATTGCAATTAGTGCTGGATGAAGGAGAGTTCCGCGTCGTAGATCATCAG
GGAAATTTTATCCTTAAGCCCTCTCCAGCCGACTTTCCCGGTTTGGCCGAAAATGAACACGCAACAATGACATTGATGTC
TCGTTTGGGATTTGACGTACCTGTGCATGGCCTGTTGAGTTTTGCACCACAATCAGAGGAAGAGCTTGAATACGCATTTG
TTATCCGGCGCTATGACCGGGATAACAAGGGGCTGCCAGTGCATCAGGAACAGCTTGATGGCGCGATGCAGATTATGGAT
AAATATGGCAAAACGGGGAATGATAACGAACAATATGTCAGCTATGAAACGCTGGCCCGATTTTTAGTTGCCCATGTTAA
CGACAACATCGCATTTAAAATCGATCTCTTTCGTCGCATTGTTTACGCCTGGTTGCTGGGTAATAACGACATGCATTTAC
GAAACTTTGGCTTAGTGTACTCCGATGGTTTGACGCCAGCGTTAGCGCCGGTGTATGACTTTGTTTCTGTCGCTCCCTAT
CCGGAATATTTTCACTCAAACTATCTGGCGCTGCCGTTATTGACGCGAGAAGAAGGCGGTCGGGAGCTGGCTCCTGGATT
TCACAGTGATTATGGCGAGTATATCGGGCAGGACTTTTTGCTGTTGGGTGAATCAATGGGGTTAGCGCCCAGATTGCTGG
AAAAACTCTTTCAGGATATACGTAAAGAAAATGCCATTGTTATGGAAACGTATGAGCAGTCCTTTATGACTCAGGATCAT
ATCCAGGCTGTGCTGCAATGCTATCGACATCGTCTGGGGCTGCTGTGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11821.47 Da        Isoelectric Point: 6.0609

>AT40579 WP_001312177.1 NZ_AP025677:183147-183458 [Escherichia coli]
MHRRVKVLLYGQVVGELSQNDSGFLFQYAPDYHGPAISISLPVAQRQFPSEMLHPYFASLAPEGWLRQRYSQIQHRDEND
LLGMLIDNGKNLLGAIQILPWEE

Download         Length: 312 bp

>AT40579 NZ_AP025677:183147-183458 [Escherichia coli]
ATGCATCGGCGAGTGAAAGTGTTGCTCTATGGTCAGGTAGTGGGTGAACTCTCTCAGAACGACAGTGGTTTTTTGTTCCA
GTATGCCCCGGATTACCACGGACCGGCAATCTCAATCAGCCTGCCCGTCGCACAGCGTCAGTTTCCCAGTGAGATGTTGC
ATCCTTATTTCGCCTCACTGGCGCCAGAAGGATGGCTGCGTCAACGCTACAGCCAGATACAGCATCGTGACGAAAACGAT
CTGCTGGGAATGCTTATTGATAACGGCAAGAATCTGCTGGGGGCCATCCAGATTTTACCTTGGGAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3K2ZTB5

Antitoxin

Source ID Structure
AlphaFold DB A0A3L3HKE2

References