Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 65823..66076 Replicon plasmid pRb3
Accession NZ_AP025676
Organism Escherichia coli strain Rb-3

Toxin (Protein)


Gene name srnB Uniprot ID A0A148HBD8
Locus tag MWM15_RS24500 Protein ID WP_001336447.1
Coordinates 65927..66076 (+) Length 50 a.a.

Antitoxin (RNA)


Gene name sok
Locus tag -
Coordinates 65823..65879 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWM15_RS24470 (61735) 61735..62481 + 747 WP_113318688.1 conjugal transfer pilus acetylase TraX -
MWM15_RS24475 (62536) 62536..63093 + 558 WP_000139329.1 fertility inhibition protein FinO -
MWM15_RS24480 (63245) 63245..63448 + 204 WP_001336517.1 hypothetical protein -
MWM15_RS24485 (64190) 64190..64651 + 462 WP_019842128.1 thermonuclease family protein -
MWM15_RS24490 (64920) 64920..65138 + 219 WP_113365647.1 hypothetical protein -
MWM15_RS24495 (65291) 65291..65728 + 438 WP_000872609.1 hypothetical protein -
- (65823) 65823..65879 - 57 NuclAT_1 - Antitoxin
- (65823) 65823..65879 - 57 NuclAT_1 - Antitoxin
- (65823) 65823..65879 - 57 NuclAT_1 - Antitoxin
- (65823) 65823..65879 - 57 NuclAT_1 - Antitoxin
MWM15_RS24500 (65927) 65927..66076 + 150 WP_001336447.1 Hok/Gef family protein Toxin
MWM15_RS24505 (66361) 66361..66618 + 258 WP_000084404.1 replication regulatory protein RepA -
MWM15_RS24510 (66723) 66723..66908 + 186 WP_012615301.1 plasmid copy control protein CopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..66920 66920


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(4-45)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5572.69 Da        Isoelectric Point: 8.7678

>T40577 WP_001336447.1 NZ_AP025676:65927-66076 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Download         Length: 150 bp

>T40577 NZ_AP025676:65927-66076 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 57 bp

>AT40577 NZ_AP025676:c65879-65823 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A148HBD8

Antitoxin

Download structure file

References