Detailed information of TA system TA040573

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4829651..4830253
• Replicon: chromosome
• Accession: NZ_AP025675
• Organism: Escherichia coli strain Rb-3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: MWM15_RS23030
• Protein ID: WP_000897305.1
• Coordinates: 4829942..4830253 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MWM15_RS23025
• Protein ID: WP_000356397.1
• Coordinates: 4829651..4829941 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWM15_RS23005 (4826153)	4826153..4827055	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
MWM15_RS23010 (4827052)	4827052..4827687	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
MWM15_RS23015 (4827684)	4827684..4828613	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
MWM15_RS23020 (4828828)	4828828..4829046	-	219	WP_001298592.1	CopG family transcriptional regulator	-
MWM15_RS23025 (4829651)	4829651..4829941	-	291	WP_000356397.1	NadS family protein	Antitoxin
MWM15_RS23030 (4829942)	4829942..4830253	-	312	WP_000897305.1	hypothetical protein	Toxin
MWM15_RS23035 (4830482)	4830482..4831390	+	909	WP_001298596.1	alpha/beta hydrolase	-
MWM15_RS23040 (4831454)	4831454..4832395	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
MWM15_RS23045 (4832440)	4832440..4832877	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
MWM15_RS23050 (4832874)	4832874..4833746	-	873	WP_000920762.1	virulence factor BrkB family protein	-
MWM15_RS23055 (4833740)	4833740..4834339	-	600	WP_001298594.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12172.16 Da
• Isoelectric Point: 9.7791

>T40573 WP_000897305.1 NZ_AP025675:c4830253-4829942 [Escherichia coli]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T40573 NZ_AP025675:c4830253-4829942 [Escherichia coli]
ATCCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT40573 WP_000356397.1 NZ_AP025675:c4829941-4829651 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT40573 NZ_AP025675:c4829941-4829651 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7