Detailed information of TA system TA040567

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4220804..4221216
• Replicon: chromosome
• Accession: NZ_AP025675
• Organism: Escherichia coli strain Rb-3

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: MWM15_RS20205
• Protein ID: WP_000132614.1
• Coordinates: 4220875..4221216 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4220804..4220880 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWM15_RS20195 (4217479)	4217479..4218948	+	1470	WP_001540814.1	type I restriction-modification system subunit M	-
MWM15_RS20200 (4218948)	4218948..4220654	+	1707	WP_001100194.1	restriction endonuclease subunit S	-
- (4220804)	4220804..4220880	-	77	NuclAT_6	-	Antitoxin
- (4220804)	4220804..4220880	-	77	NuclAT_6	-	Antitoxin
- (4220804)	4220804..4220880	-	77	NuclAT_6	-	Antitoxin
- (4220804)	4220804..4220880	-	77	NuclAT_6	-	Antitoxin
- (4220804)	4220804..4220880	-	77	NuclAT_7	-	Antitoxin
- (4220804)	4220804..4220880	-	77	NuclAT_7	-	Antitoxin
- (4220804)	4220804..4220880	-	77	NuclAT_7	-	Antitoxin
- (4220804)	4220804..4220880	-	77	NuclAT_7	-	Antitoxin
MWM15_RS20205 (4220875)	4220875..4221216	+	342	WP_000132614.1	endoribonuclease SymE	Toxin
MWM15_RS20210 (4221263)	4221263..4222426	-	1164	WP_072002093.1	DUF1524 domain-containing protein	-
MWM15_RS20215 (4222474)	4222474..4223356	-	883	Protein_3965	DUF262 domain-containing protein	-
MWM15_RS20220 (4223546)	4223546..4223623	+	78	Protein_3966	hypothetical protein	-
MWM15_RS20225 (4223762)	4223762..4224682	-	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
MWM15_RS20230 (4224867)	4224867..4226147	+	1281	WP_001298033.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheD	4204683..4223419	18736

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T40567 WP_000132614.1 NZ_AP025675:4220875-4221216 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T40567 NZ_AP025675:4220875-4221216 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT40567 NZ_AP025675:c4220880-4220804 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin