Detailed information of TA system TA040563

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3827190..3827884
• Replicon: chromosome
• Accession: NZ_AP025675
• Organism: Escherichia coli strain Rb-3

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: MWM15_RS18300
• Protein ID: WP_001263500.1
• Coordinates: 3827190..3827588 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: MWM15_RS18305
• Protein ID: WP_000554758.1
• Coordinates: 3827591..3827884 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWM15_RS18275 (3822555)	3822555..3823013	-	459	WP_001291991.1	xanthine phosphoribosyltransferase	-
MWM15_RS18280 (3823274)	3823274..3824731	+	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
MWM15_RS18285 (3824788)	3824788..3825402	-	615	WP_000602124.1	peptide chain release factor H	-
MWM15_RS18290 (3825399)	3825399..3826538	-	1140	WP_000521561.1	RNA ligase RtcB family protein	-
MWM15_RS18295 (3826728)	3826728..3827180	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
MWM15_RS18300 (3827190)	3827190..3827588	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
MWM15_RS18305 (3827591)	3827591..3827884	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
MWM15_RS18310 (3827936)	3827936..3828991	-	1056	WP_001226168.1	DNA polymerase IV	-
MWM15_RS18315 (3829062)	3829062..3829847	-	786	WP_000207578.1	putative lateral flagellar export/assembly protein LafU	-
MWM15_RS18320 (3829819)	3829819..3831531	+	1713	Protein_3595	flagellar biosynthesis protein FlhA	-
MWM15_RS18325 (3831851)	3831851..3832348	-	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3827190..3847544	20354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T40563 WP_001263500.1 NZ_AP025675:c3827588-3827190 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T40563 NZ_AP025675:c3827588-3827190 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT40563 WP_000554758.1 NZ_AP025675:c3827884-3827591 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT40563 NZ_AP025675:c3827884-3827591 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3