Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3526330..3527009 Replicon chromosome
Accession NZ_AP025675
Organism Escherichia coli strain Rb-3

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag MWM15_RS16895 Protein ID WP_000057523.1
Coordinates 3526707..3527009 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag MWM15_RS16890 Protein ID WP_000806442.1
Coordinates 3526330..3526671 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWM15_RS16880 (3522574) 3522574..3523506 - 933 WP_000883025.1 glutaminase A -
MWM15_RS16885 (3523768) 3523768..3526272 + 2505 WP_000083943.1 copper-exporting P-type ATPase CopA -
MWM15_RS16890 (3526330) 3526330..3526671 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
MWM15_RS16895 (3526707) 3526707..3527009 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MWM15_RS16900 (3527142) 3527142..3527936 + 795 WP_000365148.1 TraB/GumN family protein -
MWM15_RS16905 (3528140) 3528140..3528619 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
MWM15_RS16910 (3528643) 3528643..3529443 + 801 WP_000439798.1 hypothetical protein -
MWM15_RS16915 (3529440) 3529440..3529943 + 504 WP_000667000.1 hypothetical protein -
MWM15_RS16920 (3529981) 3529981..3531633 - 1653 WP_000771760.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T40560 WP_000057523.1 NZ_AP025675:c3527009-3526707 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T40560 NZ_AP025675:c3527009-3526707 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT40560 WP_000806442.1 NZ_AP025675:c3526671-3526330 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT40560 NZ_AP025675:c3526671-3526330 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGTCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References