Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2570886..2571448 Replicon chromosome
Accession NZ_AP025675
Organism Escherichia coli strain Rb-3

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag MWM15_RS12130 Protein ID WP_000605675.1
Coordinates 2570886..2571164 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag MWM15_RS12135 Protein ID WP_000781370.1
Coordinates 2571164..2571448 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWM15_RS12105 (2566472) 2566472..2566903 - 432 WP_000152305.1 peroxiredoxin OsmC -
MWM15_RS12110 (2567248) 2567248..2567463 + 216 WP_000495766.1 biofilm-dependent modulation protein -
MWM15_RS12115 (2567565) 2567565..2567702 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
MWM15_RS12120 (2567859) 2567859..2569556 + 1698 WP_000433462.1 malate dehydrogenase -
MWM15_RS12125 (2569690) 2569690..2570700 + 1011 WP_000642380.1 alcohol dehydrogenase AdhP -
MWM15_RS12130 (2570886) 2570886..2571164 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MWM15_RS12135 (2571164) 2571164..2571448 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
MWM15_RS12140 (2571677) 2571677..2572330 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
MWM15_RS12145 (2572323) 2572323..2573207 - 885 WP_001240581.1 formate dehydrogenase N subunit beta -
MWM15_RS12150 (2573220) 2573220..2576267 - 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T40559 WP_000605675.1 NZ_AP025675:2570886-2571164 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T40559 NZ_AP025675:2570886-2571164 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT40559 WP_000781370.1 NZ_AP025675:2571164-2571448 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT40559 NZ_AP025675:2571164-2571448 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References