Detailed information of TA system TA040535

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 123186..123607
• Replicon: plasmid pMTY20902_IncFI
• Accession: NZ_AP025663
• Organism: Escherichia coli strain TUM20902

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: M2084_RS24085
• Protein ID: WP_096937776.1
• Coordinates: 123186..123311 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	123409..123607 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M2084_RS24045 (118251)	118251..118466	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
M2084_RS24050 (118602)	118602..119249	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
M2084_RS24055 (119441)	119441..119824	-	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
M2084_RS24060 (120164)	120164..120766	+	603	WP_077248356.1	transglycosylase SLT domain-containing protein	-
M2084_RS24065 (121061)	121061..121882	-	822	WP_001234475.1	DUF932 domain-containing protein	-
M2084_RS24070 (122001)	122001..122288	-	288	WP_000107546.1	hypothetical protein	-
M2084_RS24075 (122313)	122313..122519	-	207	WP_000547965.1	hypothetical protein	-
M2084_RS24080 (122589)	122589..122885	+	297	Protein_125	hypothetical protein	-
M2084_RS24085 (123186)	123186..123311	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
M2084_RS24090 (123253)	123253..123402	-	150	Protein_127	DUF5431 family protein	-
- (123409)	123409..123607	-	199	NuclAT_0	-	Antitoxin
- (123409)	123409..123607	-	199	NuclAT_0	-	Antitoxin
- (123409)	123409..123607	-	199	NuclAT_0	-	Antitoxin
- (123409)	123409..123607	-	199	NuclAT_0	-	Antitoxin
M2084_RS24095 (123576)	123576..124338	-	763	Protein_128	plasmid SOS inhibition protein A	-
M2084_RS24100 (124335)	124335..124769	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
M2084_RS24105 (124824)	124824..126782	-	1959	Protein_130	ParB/RepB/Spo0J family partition protein	-
M2084_RS24110 (126848)	126848..127081	-	234	WP_000005990.1	DUF905 family protein	-
M2084_RS24115 (127138)	127138..127677	-	540	WP_000290801.1	single-stranded DNA-binding protein	-
M2084_RS24120 (127995)	127995..128504	+	510	WP_071600123.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..170915	170915

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T40535 WP_096937776.1 NZ_AP025663:c123311-123186 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T40535 NZ_AP025663:c123311-123186 [Escherichia coli]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT40535 NZ_AP025663:c123607-123409 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin