Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2529357..2529919 Replicon chromosome
Accession NZ_AP025662
Organism Escherichia coli strain TUM20902

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag M2084_RS12060 Protein ID WP_000605676.1
Coordinates 2529357..2529635 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag M2084_RS12065 Protein ID WP_000781370.1
Coordinates 2529635..2529919 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2084_RS12035 (2524943) 2524943..2525374 - 432 WP_000152305.1 peroxiredoxin OsmC -
M2084_RS12040 (2525719) 2525719..2525934 + 216 WP_000495766.1 biofilm-dependent modulation protein -
M2084_RS12045 (2526036) 2526036..2526173 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
M2084_RS12050 (2526330) 2526330..2528027 + 1698 WP_000433464.1 malate dehydrogenase -
M2084_RS12055 (2528161) 2528161..2529171 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
M2084_RS12060 (2529357) 2529357..2529635 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
M2084_RS12065 (2529635) 2529635..2529919 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
M2084_RS12070 (2530147) 2530147..2530800 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
M2084_RS12075 (2530793) 2530793..2531677 - 885 WP_001240565.1 formate dehydrogenase N subunit beta -
M2084_RS12080 (2531690) 2531690..2534737 - 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T40523 WP_000605676.1 NZ_AP025662:2529357-2529635 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T40523 NZ_AP025662:2529357-2529635 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCATCGACTTGCTGTGATTGATGCGGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT40523 WP_000781370.1 NZ_AP025662:2529635-2529919 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT40523 NZ_AP025662:2529635-2529919 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGTCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGATTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References