Detailed information of TA system TA040501

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 123184..123605
• Replicon: plasmid pMTY13867_IncFI
• Accession: NZ_AP025658
• Organism: Escherichia coli strain TUM13867

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: MWM17_RS24100
• Protein ID: WP_096937776.1
• Coordinates: 123184..123309 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	123407..123605 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWM17_RS24065 (118251)	118251..118466	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
MWM17_RS24070 (118602)	118602..119249	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
MWM17_RS24075 (119441)	119441..119824	-	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
MWM17_RS24080 (120164)	120164..120766	+	603	WP_077248356.1	transglycosylase SLT domain-containing protein	-
MWM17_RS24085 (121061)	121061..121882	-	822	WP_001234475.1	DUF932 domain-containing protein	-
MWM17_RS24090 (122001)	122001..122288	-	288	WP_000107546.1	hypothetical protein	-
MWM17_RS24095 (122605)	122605..122883	+	279	Protein_124	hypothetical protein	-
MWM17_RS24100 (123184)	123184..123309	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
MWM17_RS24105 (123251)	123251..123400	-	150	Protein_126	DUF5431 family protein	-
- (123407)	123407..123605	-	199	NuclAT_0	-	Antitoxin
- (123407)	123407..123605	-	199	NuclAT_0	-	Antitoxin
- (123407)	123407..123605	-	199	NuclAT_0	-	Antitoxin
- (123407)	123407..123605	-	199	NuclAT_0	-	Antitoxin
MWM17_RS24110 (123574)	123574..124336	-	763	Protein_127	plasmid SOS inhibition protein A	-
MWM17_RS24115 (124333)	124333..124767	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
MWM17_RS24120 (124822)	124822..126780	-	1959	Protein_129	ParB/RepB/Spo0J family partition protein	-
MWM17_RS24125 (126846)	126846..127079	-	234	WP_000005990.1	DUF905 family protein	-
MWM17_RS24130 (127136)	127136..127675	-	540	WP_000290801.1	single-stranded DNA-binding protein	-
MWM17_RS24135 (127993)	127993..128502	+	510	WP_071600123.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..171085	171085

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T40501 WP_096937776.1 NZ_AP025658:c123309-123184 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T40501 NZ_AP025658:c123309-123184 [Escherichia coli]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT40501 NZ_AP025658:c123605-123407 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin