Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3379880..3380442 Replicon chromosome
Accession NZ_AP025657
Organism Escherichia coli strain TUM13867

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag MWM17_RS16315 Protein ID WP_000605676.1
Coordinates 3380164..3380442 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag MWM17_RS16310 Protein ID WP_000781370.1
Coordinates 3379880..3380164 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWM17_RS16295 (3375062) 3375062..3378109 + 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -
MWM17_RS16300 (3378122) 3378122..3379006 + 885 WP_001240565.1 formate dehydrogenase N subunit beta -
MWM17_RS16305 (3378999) 3378999..3379652 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
MWM17_RS16310 (3379880) 3379880..3380164 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
MWM17_RS16315 (3380164) 3380164..3380442 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MWM17_RS16320 (3380628) 3380628..3381638 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
MWM17_RS16325 (3381772) 3381772..3383469 - 1698 WP_000433464.1 malate dehydrogenase -
MWM17_RS16330 (3383626) 3383626..3383763 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
MWM17_RS16335 (3383865) 3383865..3384080 - 216 WP_000495766.1 biofilm-dependent modulation protein -
MWM17_RS16340 (3384425) 3384425..3384856 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T40491 WP_000605676.1 NZ_AP025657:c3380442-3380164 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T40491 NZ_AP025657:c3380442-3380164 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCATCGACTTGCTGTGATTGATGCGGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT40491 WP_000781370.1 NZ_AP025657:c3380164-3379880 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT40491 NZ_AP025657:c3380164-3379880 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGTCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGATTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References