Detailed information of TA system TA040416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 290512..291135
• Replicon: chromosome
• Accession: NZ_AP025556
• Organism: Delftia lacustris strain HQS1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7T3DCK5
• Locus tag: HQS1_RS01280
• Protein ID: WP_016452144.1
• Coordinates: 290512..290793 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HQS1_RS01285
• Protein ID: WP_013800141.1
• Coordinates: 290836..291135 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQS1_RS01255 (HQS1_02550)	285868..286731	+	864	WP_016448674.1	branched-chain amino acid ABC transporter permease	-
HQS1_RS01260 (HQS1_02560)	286732..287706	+	975	WP_034366560.1	branched-chain amino acid ABC transporter permease	-
HQS1_RS01265 (HQS1_02570)	287693..288457	+	765	WP_016452146.1	ABC transporter ATP-binding protein	-
HQS1_RS01270 (HQS1_02580)	288444..289151	+	708	WP_013800138.1	ABC transporter ATP-binding protein	-
HQS1_RS01275 (HQS1_02590)	289207..290382	+	1176	WP_013800139.1	ABC transporter substrate-binding protein	-
HQS1_RS01280 (HQS1_02600)	290512..290793	+	282	WP_016452144.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HQS1_RS01285 (HQS1_02610)	290836..291135	+	300	WP_013800141.1	HigA family addiction module antitoxin	Antitoxin
HQS1_RS01290 (HQS1_02620)	291228..292001	-	774	WP_016448669.1	SDR family oxidoreductase	-
HQS1_RS01295 (HQS1_02630)	292137..292943	-	807	WP_016448668.1	IclR family transcriptional regulator	-
HQS1_RS01300 (HQS1_02640)	292988..293761	-	774	WP_016448667.1	ferredoxin--NADP reductase	-
HQS1_RS01305 (HQS1_02650)	294023..294958	-	936	WP_244387730.1	LysR family transcriptional regulator	-
HQS1_RS01310 (HQS1_02660)	295052..295843	+	792	WP_244387731.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.72 Da
• Isoelectric Point: 10.1059

>T40416 WP_016452144.1 NZ_AP025556:290512-290793 [Delftia lacustris]
MTIQSFKCPDTEQLFQQRRVRRWSSIEVVALRKLRMLHAAHVLQDLRVPPGNRLEALHGDRKGQHSIRINDQWRVCFVWT
HEGPSQLEIVDYH

Nucleotide

• Length: 282 bp

>T40416 NZ_AP025556:290512-290793 [Delftia lacustris]
ATGACGATCCAGTCATTCAAGTGCCCCGACACCGAGCAACTGTTCCAGCAGAGGCGGGTGCGTCGGTGGAGCAGCATTGA
AGTGGTTGCGCTGCGCAAGCTTCGGATGCTGCATGCCGCTCATGTGCTTCAGGACTTGAGGGTCCCGCCCGGAAACCGGC
TGGAGGCGCTGCACGGAGATCGCAAGGGGCAGCACAGCATCCGCATCAATGACCAGTGGCGCGTGTGCTTTGTGTGGACC
CATGAAGGCCCGAGTCAGCTGGAGATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10990.57 Da
• Isoelectric Point: 4.5183

>AT40416 WP_013800141.1 NZ_AP025556:290836-291135 [Delftia lacustris]
MNKPLNLPTPGEILATEFLEPMGLTQYRLAQAIHVPQTRIAAIVKNGRSITADTALRLARFFGTSVEFWMNLQSQYDADI
AREAIGDDLESIEPFALQA

Nucleotide

• Length: 300 bp

>AT40416 NZ_AP025556:290836-291135 [Delftia lacustris]
ATGAACAAACCTTTGAACCTCCCCACGCCAGGCGAAATCCTGGCCACTGAATTCCTGGAGCCCATGGGCCTGACGCAATA
CCGCCTGGCCCAGGCCATCCATGTCCCGCAGACACGCATCGCGGCCATCGTCAAGAACGGCCGCTCCATCACGGCGGATA
CGGCGCTGCGGCTGGCGCGCTTCTTTGGCACGTCCGTGGAGTTCTGGATGAATCTGCAAAGCCAGTACGACGCCGATATC
GCGCGCGAGGCCATAGGCGACGACCTGGAGTCCATCGAGCCCTTTGCTCTGCAGGCTTGA

Structures

Toxin

• AlphaFold DB: A0A7T3DCK5