Detailed information of TA system TA040401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1750889..1751391
• Replicon: chromosome
• Accession: NZ_AP025551
• Organism: Escherichia coli strain LR-50

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: QUE19_RS08275
• Protein ID: WP_000767829.1
• Coordinates: 1751137..1751391 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: QUE19_RS08270
• Protein ID: WP_001259255.1
• Coordinates: 1750889..1751140 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE19_RS08245 (1746066)	1746066..1747133	-	1068	WP_033801385.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
QUE19_RS08250 (1747133)	1747133..1748203	-	1071	WP_033801386.1	histidinol-phosphate transaminase	-
QUE19_RS08255 (1748200)	1748200..1749504	-	1305	WP_033801387.1	histidinol dehydrogenase	-
QUE19_RS08260 (1749510)	1749510..1750409	-	900	WP_137503652.1	ATP phosphoribosyltransferase	-
QUE19_RS08265 (1750555)	1750555..1750605	-	51	WP_001364200.1	his operon leader peptide	-
QUE19_RS08270 (1750889)	1750889..1751140	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
QUE19_RS08275 (1751137)	1751137..1751391	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
QUE19_RS08280 (1751474)	1751474..1752298	+	825	WP_000754737.1	SDR family oxidoreductase	-
QUE19_RS08285 (1752344)	1752344..1753273	+	930	WP_000803360.1	LysR substrate-binding domain-containing protein	-
QUE19_RS08290 (1753488)	1753488..1753550	+	63	WP_010723108.1	membrane protein YoeI	-
QUE19_RS08295 (1753540)	1753540..1754898	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
QUE19_RS08300 (1755077)	1755077..1756135	+	1059	WP_000492310.1	thiosulfate utilization transporter TsuA/YeeE	-
QUE19_RS08305 (1756149)	1756149..1756376	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T40401 WP_000767829.1 NZ_AP025551:1751137-1751391 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T40401 NZ_AP025551:1751137-1751391 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT40401 WP_001259255.1 NZ_AP025551:1750889-1751140 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT40401 NZ_AP025551:1750889-1751140 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• AlphaFold DB: A0A7U9LNR0