Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4182657..4183154 Replicon chromosome
Accession NZ_AP025546
Organism Escherichia coli strain LR-06

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QUE22_RS20540 Protein ID WP_001173343.1
Coordinates 4182657..4182830 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QUE22_RS20545 Protein ID WP_000398619.1
Coordinates 4182858..4183154 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE22_RS20530 (4179327) 4179327..4180784 + 1458 WP_094304342.1 dipeptide/tripeptide permease DtpC -
QUE22_RS20535 (4181021) 4181021..4182538 + 1518 WP_001295074.1 lysine--tRNA ligase -
QUE22_RS20540 (4182657) 4182657..4182830 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QUE22_RS20545 (4182858) 4182858..4183154 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QUE22_RS20550 (4183381) 4183381..4183653 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
QUE22_RS20555 (4183665) 4183665..4183895 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
QUE22_RS20560 (4184076) 4184076..4185707 + 1632 WP_001216477.1 sensor histidine kinase -
QUE22_RS20565 (4185704) 4185704..4186423 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4172758..4183154 10396


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T40393 WP_001173343.1 NZ_AP025546:c4182830-4182657 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T40393 NZ_AP025546:c4182830-4182657 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT40393 WP_000398619.1 NZ_AP025546:c4183154-4182858 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT40393 NZ_AP025546:c4183154-4182858 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References