Detailed information of TA system TA040392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3663490..3664184
• Replicon: chromosome
• Accession: NZ_AP025546
• Organism: Escherichia coli strain LR-06

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: QUE22_RS18025
• Protein ID: WP_001263493.1
• Coordinates: 3663490..3663888 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: QUE22_RS18030
• Protein ID: WP_000554757.1
• Coordinates: 3663891..3664184 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3659150)	3659150..3659230	-	81	NuclAT_11	-	-
- (3659150)	3659150..3659230	-	81	NuclAT_11	-	-
- (3659150)	3659150..3659230	-	81	NuclAT_11	-	-
- (3659150)	3659150..3659230	-	81	NuclAT_11	-	-
QUE22_RS17995 (3658490)	3658490..3659734	-	1245	WP_000189541.1	esterase FrsA	-
QUE22_RS18000 (3659826)	3659826..3660284	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
QUE22_RS18005 (3660545)	3660545..3662002	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
QUE22_RS18010 (3662059)	3662059..3662580	-	522	Protein_3522	peptide chain release factor H	-
QUE22_RS18015 (3662579)	3662579..3662782	-	204	Protein_3523	RtcB family protein	-
QUE22_RS18020 (3663028)	3663028..3663480	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
QUE22_RS18025 (3663490)	3663490..3663888	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
QUE22_RS18030 (3663891)	3663891..3664184	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
QUE22_RS18035 (3664236)	3664236..3665291	-	1056	WP_001226164.1	DNA polymerase IV	-
QUE22_RS18040 (3665362)	3665362..3666147	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
QUE22_RS18045 (3666119)	3666119..3667831	+	1713	Protein_3529	flagellar biosynthesis protein FlhA	-
QUE22_RS18050 (3668055)	3668055..3668552	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T40392 WP_001263493.1 NZ_AP025546:c3663888-3663490 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T40392 NZ_AP025546:c3663888-3663490 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT40392 WP_000554757.1 NZ_AP025546:c3664184-3663891 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT40392 NZ_AP025546:c3664184-3663891 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6