Detailed information of TA system TA040360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1771972..1772623
• Replicon: chromosome
• Accession: NZ_AP025524
• Organism: Mycobacterium sp. 20KCMC460

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MKCMC460_RS08290
• Protein ID: WP_236983879.1
• Coordinates: 1772273..1772623 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MKCMC460_RS08285
• Protein ID: WP_238305518.1
• Coordinates: 1771972..1772292 (-)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKCMC460_RS08265 (MKCMC460_16360)	1767813..1768238	-	426	WP_236981457.1	MazG nucleotide pyrophosphohydrolase domain-containing protein	-
MKCMC460_RS08270 (MKCMC460_16380)	1768443..1769060	-	618	WP_236981458.1	dTMP kinase	-
MKCMC460_RS08275 (MKCMC460_16390)	1769057..1770541	-	1485	WP_236981459.1	radical SAM protein	-
MKCMC460_RS08280 (MKCMC460_16400)	1770489..1771412	-	924	WP_238305517.1	hypothetical protein	-
MKCMC460_RS08285 (MKCMC460_16410)	1771972..1772292	-	321	WP_238305518.1	XRE family transcriptional regulator	Antitoxin
MKCMC460_RS08290 (MKCMC460_16420)	1772273..1772623	-	351	WP_236983879.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MKCMC460_RS08295 (MKCMC460_16430)	1772688..1773743	-	1056	WP_238305519.1	site-specific integrase	-
MKCMC460_RS08300 (MKCMC460_16440)	1773688..1774017	-	330	WP_238305520.1	Arm DNA-binding domain-containing protein	-
MKCMC460_RS08305 (MKCMC460_16450)	1774014..1774196	-	183	WP_238305522.1	helix-turn-helix domain-containing protein	-
MKCMC460_RS08310 (MKCMC460_16460)	1774203..1775045	-	843	WP_238305524.1	helix-turn-helix domain-containing protein	-
MKCMC460_RS08315 (MKCMC460_16470)	1775359..1775730	-	372	WP_236981463.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1763635..1779416	15781

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (10-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-91)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13015.82 Da
• Isoelectric Point: 4.8838

>T40360 WP_236983879.1 NZ_AP025524:c1772623-1772273 [Mycobacterium sp. 20KCMC460]
IILLEEVEAWYFTLDSYTTAAVTGAIDLLKLEGPSLGRPTVDRVKASKFHSMKELRPVGTSIRILFIFDPERQAILLLGG
DKAGNWKSWYDKNIPKAEQRYENWLAGSDDDGTTLA

Nucleotide

• Length: 351 bp

>T40360 NZ_AP025524:c1772623-1772273 [Mycobacterium sp. 20KCMC460]
ATAATCCTCCTTGAGGAGGTCGAAGCCTGGTACTTCACGCTCGATAGCTACACGACGGCCGCCGTTACCGGCGCCATCGA
CCTGCTTAAGCTCGAAGGACCAAGCTTGGGTCGTCCAACGGTGGACAGAGTCAAGGCATCGAAGTTTCACAGCATGAAGG
AGCTGCGGCCGGTCGGCACCAGCATCCGGATTCTGTTCATCTTCGACCCAGAGCGGCAGGCAATCCTGTTGCTCGGCGGC
GACAAGGCGGGCAACTGGAAGAGCTGGTACGACAAGAACATTCCGAAAGCTGAGCAGCGCTACGAGAACTGGCTGGCAGG
AAGTGACGACGATGGCACGACACTGGCGTGA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11533.01 Da
• Isoelectric Point: 6.9899

>AT40360 WP_238305518.1 NZ_AP025524:c1772292-1771972 [Mycobacterium sp. 20KCMC460]
MARHWREIRAEAVASGHVDPARADAARKEMYDAVQAQRLADIRKAHHARQADVAALMGVSQAWVSRLEGGDLSHTELGTL
QSYVAALGGSLRVVAEFGERIVELTT

Nucleotide

• Length: 321 bp

>AT40360 NZ_AP025524:c1772292-1771972 [Mycobacterium sp. 20KCMC460]
ATGGCACGACACTGGCGTGAAATCCGCGCTGAGGCGGTTGCGTCGGGGCACGTTGACCCCGCACGCGCGGATGCCGCACG
CAAAGAGATGTACGACGCCGTCCAGGCGCAGCGCCTCGCCGACATCCGCAAGGCTCATCACGCCCGTCAGGCCGACGTCG
CCGCGCTCATGGGTGTGTCGCAGGCCTGGGTGTCCAGGCTGGAGGGCGGCGACCTGTCCCATACCGAGTTGGGCACGCTG
CAGTCGTACGTGGCTGCGCTCGGCGGGAGTCTAAGGGTCGTCGCAGAGTTTGGCGAGAGGATCGTGGAGCTAACCACTTG
A