Detailed information of TA system TA040332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-MqsA
• Location: 1948307..1948940
• Replicon: chromosome
• Accession: NZ_AP025503
• Organism: Vibrio pelagius strain ATCC 25916

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: vsple_RS08355
• Protein ID: WP_261881723.1
• Coordinates: 1948608..1948940 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: vsple_RS08350
• Protein ID: WP_255230510.1
• Coordinates: 1948307..1948621 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
vsple_RS08315	1943423..1944151	-	729	WP_261881718.1	glycosyltransferase	-
vsple_RS08320	1944521..1944799	+	279	WP_255230512.1	50S ribosomal protein L25	-
vsple_RS08325	1945085..1945450	-	366	WP_261881719.1	VOC family protein	-
vsple_RS08330	1945498..1945611	-	114	Protein_1577	DUF3265 domain-containing protein	-
vsple_RS08335	1945577..1946215	-	639	WP_261881720.1	DJ-1/PfpI family protein	-
vsple_RS08340	1946339..1947112	-	774	WP_261881721.1	phosphotransferase	-
vsple_RS08345	1947248..1947757	-	510	WP_261881722.1	hypothetical protein	-
vsple_RS08350	1948307..1948621	-	315	WP_255230510.1	DNA-binding transcriptional regulator	Antitoxin
vsple_RS08355	1948608..1948940	-	333	WP_261881723.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
vsple_RS08360	1949158..1949460	-	303	WP_255230509.1	type II toxin-antitoxin system RelE/ParE family toxin	-
vsple_RS08365	1949448..1949705	-	258	WP_261881724.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
vsple_RS08370	1949787..1949876	-	90	WP_261883154.1	DUF3265 domain-containing protein	-
vsple_RS08375	1949889..1950107	+	219	Protein_1586	IS110 family transposase	-
vsple_RS08380	1950130..1950456	-	327	WP_261881725.1	hypothetical protein	-
vsple_RS08385	1950596..1951749	+	1154	Protein_1588	IS110 family transposase	-
vsple_RS08390	1952065..1952157	-	93	WP_261881726.1	DUF3265 domain-containing protein	-
vsple_RS08395	1952172..1952981	-	810	WP_047111987.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1945577..1959745	14168

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (4-104)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12927.78 Da
• Isoelectric Point: 10.0960

>T40332 WP_261881723.1 NZ_AP025503:c1948940-1948608 [Vibrio pelagius]
MKSVFVESTIFEKYRSEYLSDEEFRLFQAELMSNPKQGDVIQGTGGLRKVRFASKGKGKRGGSRVIYYFLDEKRRFYLLT
IYGKNEMSDLTADQKKQLKAFMEAWRNEQS

Nucleotide

• Length: 333 bp

>T40332 NZ_AP025503:c1948940-1948608 [Vibrio pelagius]
ATGAAAAGTGTATTCGTCGAATCAACTATATTTGAAAAGTACCGTAGTGAATACCTCAGTGATGAAGAGTTTAGACTTTT
CCAAGCTGAGTTAATGTCCAACCCGAAACAGGGGGACGTAATTCAAGGTACAGGTGGTTTGCGAAAGGTTCGATTTGCAA
GTAAAGGTAAAGGCAAGCGTGGTGGCTCTCGCGTTATCTATTACTTCCTCGATGAAAAGCGTCGTTTCTACTTGCTGACG
ATTTACGGCAAGAATGAAATGTCGGACTTAACCGCAGACCAAAAGAAACAACTAAAGGCTTTTATGGAGGCGTGGCGTAA
TGAGCAATCGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11730.21 Da
• Isoelectric Point: 6.0792

>AT40332 WP_255230510.1 NZ_AP025503:c1948621-1948307 [Vibrio pelagius]
MSNRDLFAELSSALVEAKEHSEGKLTLKTYQVNDISELNISPDEIVSIREQFNMSRGVFARLLHTSSRTLENWEQGRSAP
NGQAVTLLKLVQRHPETLSHIAEL

Nucleotide

• Length: 315 bp

>AT40332 NZ_AP025503:c1948621-1948307 [Vibrio pelagius]
ATGAGCAATCGTGATTTATTTGCAGAGTTAAGCTCAGCACTTGTTGAGGCTAAAGAGCATTCAGAGGGCAAGCTTACCCT
TAAAACTTATCAAGTTAACGACATCAGTGAGTTAAATATCTCACCTGATGAAATCGTGAGCATTCGAGAGCAGTTTAATA
TGTCTCGTGGTGTTTTTGCTCGTTTGTTGCATACTTCATCTCGTACACTAGAGAACTGGGAACAAGGGCGTAGCGCACCA
AATGGGCAAGCTGTAACCCTTTTAAAACTGGTTCAGCGTCATCCAGAAACTCTGTCGCATATCGCTGAGCTATAA