Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1227925..1228661 Replicon chromosome
Accession NZ_AP025472
Organism Vibrio cortegadensis strain CECT 7227

Toxin (Protein)


Gene name higB Uniprot ID A0A1E5CUI4
Locus tag OCV39_RS05625 Protein ID WP_017053844.1
Coordinates 1227925..1228236 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1E5CUK5
Locus tag OCV39_RS05630 Protein ID WP_017053843.1
Coordinates 1228233..1228661 (+) Length 143 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
OCV39_RS05610 1223132..1223578 + 447 WP_261889215.1 GNAT family N-acetyltransferase -
OCV39_RS05615 1224867..1225247 + 381 WP_130350976.1 MmcQ/YjbR family DNA-binding protein -
OCV39_RS05620 1225442..1225570 - 129 WP_261383053.1 hypothetical protein -
OCV39_RS05625 1227925..1228236 + 312 WP_017053844.1 type II toxin-antitoxin system HigB family toxin Toxin
OCV39_RS05630 1228233..1228661 + 429 WP_017053843.1 helix-turn-helix domain-containing protein Antitoxin
OCV39_RS05635 1228814..1229602 + 789 WP_261889216.1 hypothetical protein -
OCV39_RS05640 1230299..1231132 + 834 WP_261889217.1 DUF2971 domain-containing protein -
OCV39_RS05645 1231147..1231239 + 93 Protein_1079 DUF3265 domain-containing protein -
OCV39_RS05650 1231279..1231926 + 648 WP_261889218.1 hypothetical protein -
OCV39_RS05655 1232073..1232567 + 495 WP_261889219.1 GNAT family N-acetyltransferase -
OCV39_RS05660 1232720..1233193 + 474 WP_261889220.1 GrpB family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integron - - 1200955..1350280 149325


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-137)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12135.01 Da        Isoelectric Point: 9.7599

>T40300 WP_017053844.1 NZ_AP025472:1227925-1228236 [Vibrio cortegadensis]
MHVISRKPFADAANKYPNDSLAIESLYKTLKNNNFTDPLAMKNVFPSLDNFKYKDKWYVIDIGGNNLRLMAFIEFRDSRI
FVKHIVPHDEYDKLCKKYAKESK

Download         Length: 312 bp

>T40300 NZ_AP025472:1227925-1228236 [Vibrio cortegadensis]
ATGCACGTAATTTCAAGAAAGCCATTCGCAGATGCGGCGAATAAATATCCAAATGATTCGCTTGCGATCGAGTCACTTTA
TAAGACTCTGAAAAACAACAATTTCACAGATCCATTGGCAATGAAAAATGTTTTTCCTAGCCTAGATAACTTTAAGTACA
AAGATAAGTGGTATGTGATTGATATTGGTGGCAACAATCTACGCCTAATGGCGTTTATAGAGTTTCGAGACAGCCGCATT
TTTGTTAAGCATATTGTGCCTCATGATGAATACGACAAACTGTGTAAGAAGTATGCAAAGGAGTCAAAATGA

Antitoxin


Download         Length: 143 a.a.        Molecular weight: 16069.12 Da        Isoelectric Point: 4.2113

>AT40300 WP_017053843.1 NZ_AP025472:1228233-1228661 [Vibrio cortegadensis]
MKNFTEVKSTALELFSMASFLGHISTEEEYHQALELMEELIEEYDLYKPLIEVLSHSIEIWEDEADEFAQFNASINSLDS
GAATLRTLMDQYQLKADDLKDVIGGKSLVSMVLNGSRQLTKDHIQAISDKFNVSPALFFTRA

Download         Length: 429 bp

>AT40300 NZ_AP025472:1228233-1228661 [Vibrio cortegadensis]
ATGAAAAACTTTACTGAAGTGAAATCAACAGCTTTGGAACTGTTTTCAATGGCAAGTTTTTTGGGACATATCTCAACAGA
AGAAGAGTACCATCAAGCTTTAGAGCTAATGGAAGAGTTAATCGAAGAGTATGATTTATACAAACCGTTGATCGAAGTGT
TATCTCATTCAATCGAAATTTGGGAAGATGAAGCAGACGAATTTGCACAATTCAATGCTTCTATTAACAGTTTAGATAGC
GGTGCCGCCACATTGAGAACATTGATGGATCAGTATCAACTTAAAGCTGATGACCTAAAAGATGTAATAGGTGGTAAGAG
CCTAGTATCAATGGTTTTAAATGGTTCAAGACAACTTACAAAAGATCACATTCAAGCTATTTCTGATAAATTTAATGTGA
GTCCGGCATTGTTCTTCACACGCGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1E5CUI4

Antitoxin

Source ID Structure
AlphaFold DB A0A1E5CUK5

References