Detailed information of TA system TA040290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1219971..1220707
• Replicon: chromosome
• Accession: NZ_AP025461
• Organism: Vibrio atlanticus strain CECT 7223

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1C3IQK2
• Locus tag: OCV30_RS21145
• Protein ID: WP_065678974.1
• Coordinates: 1220396..1220707 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1C3IQP4
• Locus tag: OCV30_RS21140
• Protein ID: WP_065678975.1
• Coordinates: 1219971..1220399 (-)
• Length: 143 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCV30_RS21120	1215815..1216477	-	663	WP_244499036.1	M23 family metallopeptidase	-
OCV30_RS21125	1216610..1217047	-	438	WP_065678978.1	hypothetical protein	-
OCV30_RS21130	1217613..1218662	-	1050	Protein_1066	IS3 family transposase	-
OCV30_RS21135	1218826..1219713	+	888	WP_065678976.1	site-specific integrase	-
OCV30_RS21140	1219971..1220399	-	429	WP_065678975.1	helix-turn-helix domain-containing protein	Antitoxin
OCV30_RS21145	1220396..1220707	-	312	WP_065678974.1	type II toxin-antitoxin system HigB family toxin	Toxin
OCV30_RS21150	1220892..1221128	+	237	Protein_1070	IS110 family transposase	-
OCV30_RS21155	1221170..1221679	+	510	Protein_1071	transposase	-
OCV30_RS21160	1221934..1222707	-	774	WP_065678972.1	NERD domain-containing protein	-
OCV30_RS21165	1222934..1223821	+	888	WP_261879041.1	site-specific integrase	-
OCV30_RS21170	1223818..1224870	+	1053	Protein_1074	transposase	-
OCV30_RS21175	1225146..1225442	-	297	WP_050650569.1	hypothetical protein	-
OCV30_RS21180	1225525..1225617	-	93	WP_074544343.1	DUF3265 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1205815..1230573	24758
	-	inside	IScluster/Tn	-	-	1217565..1229524	11959

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (89-137)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12095.95 Da
• Isoelectric Point: 9.8657

>T40290 WP_065678974.1 NZ_AP025461:c1220707-1220396 [Vibrio atlanticus]
MHVISRKPFSDAAKKYPNDAGAIDALYKSLKNNNFSNPLDMKNVYPSLDNFKYKDKWYVLDIGGNNLRLMTFIEFRDNRM
FVKHIVPHAEYDKLCAKYVKESK

Nucleotide

• Length: 312 bp

>T40290 NZ_AP025461:c1220707-1220396 [Vibrio atlanticus]
ATGCACGTAATATCAAGAAAACCATTTAGTGATGCAGCGAAAAAATATCCGAATGATGCTGGGGCAATTGATGCACTATA
TAAATCATTAAAAAATAATAACTTTTCAAATCCACTGGATATGAAAAATGTTTACCCAAGCTTAGATAACTTTAAGTATA
AAGATAAATGGTATGTTCTTGATATCGGTGGGAATAATTTACGTTTAATGACCTTTATCGAATTTCGAGATAATAGAATG
TTTGTTAAGCATATTGTTCCTCATGCAGAATATGACAAGTTATGTGCCAAATACGTAAAGGAGTCGAAATGA

Antitoxin

Protein

• Length: 143 a.a.
• Molecular weight: 16204.43 Da
• Isoelectric Point: 4.2423

>AT40290 WP_065678975.1 NZ_AP025461:c1220399-1219971 [Vibrio atlanticus]
MKNFNQVKSTALQLFSMASFVGHISTEEEYAEALELMEDLIEEYDIYKPLIEVLEVSIERWEDRADEFAEFNSRIENLDD
GVATLRVLMDQYQLKADDLKDVIGGKSLVSMILNGSRKLTKEHIQAISSKYNISPALFFGCV

Nucleotide

• Length: 429 bp

>AT40290 NZ_AP025461:c1220399-1219971 [Vibrio atlanticus]
ATGAAAAATTTTAATCAAGTTAAATCAACAGCATTACAACTATTTTCAATGGCTAGTTTTGTTGGTCATATTTCAACAGA
AGAAGAATATGCTGAAGCCTTGGAGTTAATGGAAGATCTTATTGAAGAATACGATATTTACAAACCATTAATTGAGGTTT
TGGAAGTATCTATTGAGCGTTGGGAAGACCGTGCAGATGAATTTGCAGAGTTTAATTCCCGTATTGAGAATTTAGATGAT
GGAGTGGCAACACTGCGTGTATTAATGGATCAGTATCAACTTAAAGCTGATGACCTAAAAGATGTAATTGGTGGTAAAAG
TTTGGTTTCAATGATCCTTAACGGTTCTCGCAAACTAACAAAAGAGCATATTCAAGCTATTTCATCTAAATATAATATCA
GCCCTGCATTGTTTTTTGGCTGCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A1C3IQK2

Antitoxin

• AlphaFold DB: A0A1C3IQP4