Detailed information of TA system TA040258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1975348..1976006
• Replicon: chromosome
• Accession: NZ_AP025334
• Organism: Phytobacter diazotrophicus strain TA9734

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: L6445_RS09240
• Protein ID: WP_125124251.1
• Coordinates: 1975348..1975701 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A348DBJ5
• Locus tag: L6445_RS09245
• Protein ID: WP_071196363.1
• Coordinates: 1975704..1976006 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6445_RS09225 (1971802)	1971802..1973256	-	1455	WP_041851859.1	AMP nucleosidase	-
L6445_RS09230 (1973450)	1973450..1974379	-	930	WP_232034419.1	MDR family oxidoreductase	-
L6445_RS09235 (1974309)	1974309..1975129	-	821	Protein_1807	TetR family transcriptional regulator C-terminal domain-containing protein	-
L6445_RS09240 (1975348)	1975348..1975701	+	354	WP_125124251.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L6445_RS09245 (1975704)	1975704..1976006	+	303	WP_071196363.1	XRE family transcriptional regulator	Antitoxin
L6445_RS09250 (1976083)	1976083..1977033	+	951	WP_041851855.1	L,D-transpeptidase	-
L6445_RS09255 (1977126)	1977126..1977314	-	189	WP_041851854.1	YjfB family protein	-
L6445_RS09265 (1977912)	1977912..1978829	+	918	WP_041851853.1	nitrogen assimilation transcriptional regulator NAC	-
L6445_RS09270 (1978931)	1978931..1979881	+	951	WP_041851852.1	HTH-type transcriptional regulator Cbl	-
L6445_RS09275 (1979933)	1979933..1980409	-	477	WP_071196365.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1973450..2031395	57945

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13585.60 Da
• Isoelectric Point: 9.4816

>T40258 WP_125124251.1 NZ_AP025334:1975348-1975701 [Phytobacter diazotrophicus]
VWEIKTTDRFDEWLIAQNDHTRASVLAALIVLRTKGPELPRPWADTIKGARYSNMKELRIQCRGEPIRPFFAFDPRRAAI
VLCAGSKAGNEKRFYAEMISRAEQEYANYLKKPEKEE

Nucleotide

• Length: 354 bp

>T40258 NZ_AP025334:1975348-1975701 [Phytobacter diazotrophicus]
GTGTGGGAAATTAAAACAACGGACAGGTTTGATGAGTGGTTAATCGCTCAAAATGATCACACGCGTGCGAGCGTTTTGGC
GGCGCTGATCGTGTTGCGAACGAAAGGCCCCGAATTGCCCAGACCCTGGGCCGACACGATAAAAGGGGCGCGCTACAGCA
ATATGAAGGAGCTGCGAATTCAGTGTCGTGGGGAGCCGATACGCCCTTTTTTCGCTTTTGATCCCAGACGGGCGGCTATT
GTGTTGTGCGCAGGAAGTAAAGCTGGAAATGAAAAACGTTTCTACGCCGAAATGATTTCGCGAGCTGAGCAGGAATATGC
AAACTATCTGAAAAAACCAGAGAAAGAGGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11067.80 Da
• Isoelectric Point: 6.2312

>AT40258 WP_071196363.1 NZ_AP025334:1975704-1976006 [Phytobacter diazotrophicus]
MGRTLEQLLADEKPEVVAQAESMATETLLNIHLAELREKVQKTQVEMAHALGIRQPTVAGMEKPGRDVKLSTLKRYVEAT
GGKLRLDIELPDGSHYAFVV

Nucleotide

• Length: 303 bp

>AT40258 NZ_AP025334:1975704-1976006 [Phytobacter diazotrophicus]
ATGGGCAGAACGCTGGAACAACTGCTTGCGGATGAAAAACCAGAGGTTGTGGCGCAGGCTGAGTCAATGGCGACTGAGAC
TCTCCTGAACATTCACCTGGCCGAACTGCGTGAGAAAGTGCAAAAAACGCAGGTGGAAATGGCGCATGCACTGGGGATAC
GGCAACCTACGGTGGCGGGAATGGAAAAACCGGGTCGCGATGTAAAACTGTCGACGCTTAAGCGCTACGTTGAAGCCACT
GGCGGTAAGCTTCGTCTTGATATCGAACTGCCTGATGGGTCGCATTACGCCTTCGTTGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A348DBJ5