Detailed information of TA system TA040222

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 2691161..2691498
• Replicon: chromosome
• Accession: NZ_AP025270
• Organism: Enterococcus faecalis strain K-4

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: E2Z0W4
• Locus tag: EFK4_RS12895
• Protein ID: WP_002381035.1
• Coordinates: 2691161..2691304 (+)
• Length: 48 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	2691236..2691498 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFK4_RS12875	2686283..2686498	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
EFK4_RS12880	2686637..2687629	+	993	WP_002354765.1	biotin--[acetyl-CoA-carboxylase] ligase	-
EFK4_RS12885	2687893..2688522	-	630	WP_002354763.1	lytic polysaccharide monooxygenase	-
EFK4_RS12890	2689215..2690831	+	1617	WP_002381036.1	phosphatase PAP2/LCP family protein	-
EFK4_RS12895	2691161..2691304	+	144	WP_002381035.1	putative holin-like toxin	Toxin
-	2691236..2691498	-	263	-	-	Antitoxin
EFK4_RS12900	2691499..2696190	-	4692	WP_236919338.1	WxL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (14-47)

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5202.20 Da
• Isoelectric Point: 10.0041

>T40222 WP_002381035.1 NZ_AP025270:2691161-2691304 [Enterococcus faecalis]
MNVSTKIYERRGLLSIAEALALMISFGSFIATLIFGILEAVKENNKK

Nucleotide

• Length: 144 bp

>T40222 NZ_AP025270:2691161-2691304 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTAAAAGAAAACAATAAAAAATAA

Antitoxin

Nucleotide

• Length: 263 bp

>AT40222 NZ_AP025270:c2691498-2691236 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGATGGTCGTTGTGCTACA
ATGGTAATGAAAAGAGAGGTATGCGCCAACATATCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATT
TATAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTTTTCTTTTACAGCCTCTAGGATTCCGAAGATT
AACGTTGCGATAAAAGAACCGAA

Structures

Toxin

• AlphaFold DB: A0A2Z6BWH2

Antitoxin