Detailed information of TA system TA040215

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-ratA/-
• Location: 2612122..2612385
• Replicon: chromosome
• Accession: NZ_AP025270
• Organism: Enterococcus faecalis strain K-4

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: S4BYM2
• Locus tag: EFK4_RS12555
• Protein ID: WP_002392696.1
• Coordinates: 2612242..2612385 (-)
• Length: 48 a.a.

Antitoxin (RNA)

Gene name	ratA
Locus tag	-
Coordinates	2612122..2612309 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFK4_RS12540	2607583..2610327	+	2745	WP_002406717.1	glycosyl hydrolase family 65 protein	-
EFK4_RS12545	2610342..2610992	+	651	WP_002354875.1	beta-phosphoglucomutase	-
EFK4_RS12550	2611407..2611925	+	519	WP_002354874.1	PBECR4 domain-containing protein	-
-	2612122..2612309	+	188	-	-	Antitoxin
EFK4_RS12555	2612242..2612385	-	144	WP_002392696.1	type I toxin-antitoxin system toxin PepG1	Toxin
EFK4_RS14090	2612793..2612918	-	126	WP_002383214.1	hypothetical protein	-
EFK4_RS12560	2613123..2613329	+	207	WP_224561207.1	CPBP family intramembrane metalloprotease	-
EFK4_RS12565	2613382..2614353	-	972	WP_002381060.1	ribose-phosphate diphosphokinase	-
EFK4_RS12570	2614528..2614965	-	438	WP_002354871.1	peptide-methionine (R)-S-oxide reductase MsrB	-
EFK4_RS12575	2615098..2615652	-	555	WP_002354869.1	Maf family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (14-47)

Sequences

Toxin

Protein

• Length: 48 a.a.
• Molecular weight: 5297.43 Da
• Isoelectric Point: 10.6867

>T40215 WP_002392696.1 NZ_AP025270:c2612385-2612242 [Enterococcus faecalis]
MSVLPKFTERRGLLSAYETIQTILGFGMFTIALIALIVKLLKNDNKK

Nucleotide

• Length: 144 bp

>T40215 NZ_AP025270:c2612385-2612242 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAATAAAAAATAA

Antitoxin

Nucleotide

• Length: 188 bp

>AT40215 NZ_AP025270:2612122-2612309 [Enterococcus faecalis]
TGTGCTATAATGAAAACGAAAAGAGAGATATGCTTCAACATACCTCTCTGATGCAGAGCCGTTTAAGACGGTGACTTTTT
GAATTATTTAAAAATAACCGTACTCGGTCAAAGTTGACGGTTATTTTTTATTGTCATTTTTAAGCAATTTCACAATCAGT
GCAATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: S4BYM2

Antitoxin