Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 106636..107363 | Replicon | plasmid pOCUhvKP1 |
| Accession | NZ_AP025247 | ||
| Organism | Klebsiella pneumoniae strain OCU_hvKP1 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A7W3D9W1 |
| Locus tag | L6440_RS26660 | Protein ID | WP_011251285.1 |
| Coordinates | 106636..106947 (+) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | L6440_RS26665 | Protein ID | WP_011251286.1 |
| Coordinates | 106944..107363 (+) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| L6440_RS26620 (OCUBac04_51370) | 101697..102212 | - | 516 | WP_041937820.1 | hypothetical protein | - |
| L6440_RS26625 (OCUBac04_51380) | 102320..102895 | - | 576 | WP_074424927.1 | hypothetical protein | - |
| L6440_RS26630 (OCUBac04_51390) | 103012..103968 | - | 957 | WP_011251280.1 | DsbA family protein | - |
| L6440_RS26635 (OCUBac04_51400) | 104028..104369 | - | 342 | WP_011251281.1 | hypothetical protein | - |
| L6440_RS26640 (OCUBac04_51410) | 104383..104694 | - | 312 | WP_011251282.1 | hypothetical protein | - |
| L6440_RS26645 (OCUBac04_51420) | 104711..105160 | - | 450 | WP_011251283.1 | thioredoxin fold domain-containing protein | - |
| L6440_RS26655 (OCUBac04_51450) | 105994..106431 | + | 438 | Protein_114 | DDE-type integrase/transposase/recombinase | - |
| L6440_RS26660 (OCUBac04_51460) | 106636..106947 | + | 312 | WP_011251285.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| L6440_RS26665 (OCUBac04_51470) | 106944..107363 | + | 420 | WP_011251286.1 | helix-turn-helix domain-containing protein | Antitoxin |
| L6440_RS26670 (OCUBac04_51480) | 107510..108478 | + | 969 | WP_011251287.1 | IS5 family transposase | - |
| L6440_RS26675 (OCUBac04_51490) | 108630..109283 | + | 654 | WP_011251259.1 | class I SAM-dependent methyltransferase | - |
| L6440_RS26680 | 109353..109619 | - | 267 | Protein_119 | IS3 family transposase | - |
| L6440_RS26685 | 109630..110318 | - | 689 | Protein_120 | IS1 family transposase | - |
| L6440_RS26690 (OCUBac04_51510) | 110467..110790 | - | 324 | WP_004902375.1 | hypothetical protein | - |
| L6440_RS26695 (OCUBac04_51520) | 110934..111896 | - | 963 | WP_004902373.1 | DEAD/DEAH box helicase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA | 1..215041 | 215041 | |
| - | inside | IScluster/Tn | - | - | 105162..113545 | 8383 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12346.12 Da Isoelectric Point: 9.7248
>T40194 WP_011251285.1 NZ_AP025247:106636-106947 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE
Download Length: 312 bp
>T40194 NZ_AP025247:106636-106947 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15377.46 Da Isoelectric Point: 4.4420
>AT40194 WP_011251286.1 NZ_AP025247:106944-107363 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL
Download Length: 420 bp
>AT40194 NZ_AP025247:106944-107363 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|