Detailed information of TA system TA040133

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2759290..2759892
• Replicon: chromosome
• Accession: NZ_AP025214
• Organism: Escherichia coli strain 2017.09.02CC

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: LPH32_RS13535
• Protein ID: WP_000897305.1
• Coordinates: 2759581..2759892 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LPH32_RS13530
• Protein ID: WP_000356397.1
• Coordinates: 2759290..2759580 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LPH32_RS13510 (2755792)	2755792..2756694	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
LPH32_RS13515 (2756691)	2756691..2757326	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LPH32_RS13520 (2757323)	2757323..2758252	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
LPH32_RS13525 (2758467)	2758467..2758685	-	219	WP_001298592.1	CopG family transcriptional regulator	-
LPH32_RS13530 (2759290)	2759290..2759580	-	291	WP_000356397.1	NadS family protein	Antitoxin
LPH32_RS13535 (2759581)	2759581..2759892	-	312	WP_000897305.1	hypothetical protein	Toxin
LPH32_RS13540 (2760121)	2760121..2761029	+	909	WP_053290819.1	alpha/beta hydrolase	-
LPH32_RS13545 (2761093)	2761093..2762034	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LPH32_RS13550 (2762079)	2762079..2762516	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
LPH32_RS13555 (2762513)	2762513..2763385	-	873	WP_000920762.1	virulence factor BrkB family protein	-
LPH32_RS13560 (2763379)	2763379..2763978	-	600	WP_001314324.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T40133 WP_000897305.1 NZ_AP025214:c2759892-2759581 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T40133 NZ_AP025214:c2759892-2759581 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT40133 WP_000356397.1 NZ_AP025214:c2759580-2759290 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT40133 NZ_AP025214:c2759580-2759290 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7