Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 667454..668016 Replicon chromosome
Accession NZ_AP025214
Organism Escherichia coli strain 2017.09.02CC

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag LPH32_RS03420 Protein ID WP_000605676.1
Coordinates 667454..667732 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag LPH32_RS03425 Protein ID WP_000781370.1
Coordinates 667732..668016 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH32_RS03395 (663040) 663040..663471 - 432 WP_000152305.1 peroxiredoxin OsmC -
LPH32_RS03400 (663816) 663816..664031 + 216 WP_000495766.1 biofilm-dependent modulation protein -
LPH32_RS03405 (664133) 664133..664270 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
LPH32_RS03410 (664427) 664427..666124 + 1698 WP_001357345.1 malate dehydrogenase -
LPH32_RS03415 (666258) 666258..667268 + 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
LPH32_RS03420 (667454) 667454..667732 + 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LPH32_RS03425 (667732) 667732..668016 + 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
LPH32_RS03430 (668067) 668067..668720 - 654 WP_000045648.1 formate dehydrogenase-N subunit gamma -
LPH32_RS03435 (668713) 668713..669597 - 885 WP_001240582.1 formate dehydrogenase N subunit beta -
LPH32_RS03440 (669610) 669610..672657 - 3048 WP_015912497.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T40125 WP_000605676.1 NZ_AP025214:667454-667732 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T40125 NZ_AP025214:667454-667732 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT40125 WP_000781370.1 NZ_AP025214:667732-668016 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT40125 NZ_AP025214:667732-668016 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCGGCGCTCACTCCGGAAATGGCGA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGCCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References